ID A0A1H6FFH8_9GAMM Unreviewed; 421 AA.
AC A0A1H6FFH8;
DT 25-APR-2018, integrated into UniProtKB/TrEMBL.
DT 25-APR-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=NH(3)-dependent NAD(+) synthetase {ECO:0000256|RuleBase:RU003812};
DE EC=6.3.1.5 {ECO:0000256|RuleBase:RU003812};
GN Name=nadE {ECO:0000313|EMBL:SEH08393.1};
GN ORFNames=MBHS_04285 {ECO:0000313|EMBL:SEH08393.1};
OS Candidatus Venteria ishoeyi.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Thiotrichales;
OC Thiotrichaceae; Venteria.
OX NCBI_TaxID=1899563 {ECO:0000313|EMBL:SEH08393.1, ECO:0000313|Proteomes:UP000236724};
RN [1] {ECO:0000313|EMBL:SEH08393.1, ECO:0000313|Proteomes:UP000236724}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MBHS1 {ECO:0000313|EMBL:SEH08393.1};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) +
CC NAD(+); Xref=Rhea:RHEA:21188, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.1.5;
CC Evidence={ECO:0000256|RuleBase:RU003812};
CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004790}.
CC -!- SIMILARITY: Belongs to the IlvD/Edd family.
CC {ECO:0000256|ARBA:ARBA00006486}.
CC -!- SIMILARITY: Belongs to the NAD synthetase family.
CC {ECO:0000256|RuleBase:RU003811}.
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DR EMBL; FMSV02000549; SEH08393.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H6FFH8; -.
DR UniPathway; UPA00253; UER00333.
DR Proteomes; UP000236724; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004359; F:glutaminase activity; IEA:InterPro.
DR GO; GO:0016836; F:hydro-lyase activity; IEA:InterPro.
DR GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR GO; GO:0008795; F:NAD+ synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009435; P:NAD biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR InterPro; IPR037237; IlvD/EDD_N.
DR InterPro; IPR022310; NAD/GMP_synthase.
DR InterPro; IPR003694; NAD_synthase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR NCBIfam; TIGR00552; nadE; 1.
DR PANTHER; PTHR23090:SF9; GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE; 1.
DR PANTHER; PTHR23090; NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE; 1.
DR Pfam; PF00920; ILVD_EDD; 1.
DR Pfam; PF02540; NAD_synthase; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF143975; IlvD/EDD N-terminal domain-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU003811};
KW Ligase {ECO:0000256|RuleBase:RU003811, ECO:0000313|EMBL:SEH08393.1};
KW Lyase {ECO:0000256|ARBA:ARBA00023239}; Membrane {ECO:0000256|SAM:Phobius};
KW NAD {ECO:0000256|RuleBase:RU003811};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU003811};
KW Reference proteome {ECO:0000313|Proteomes:UP000236724};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 82..341
FT /note="NAD/GMP synthase"
FT /evidence="ECO:0000259|Pfam:PF02540"
SQ SEQUENCE 421 AA; 47160 MW; FF3411E6688B0804 CRC64;
MLEQDIKPRD IMTRAAFENA MVLLMALGGS TNAVLHLIAM ARSVDVELSI DDFQAISDRI
PFIADLKPSG RYVMEDLHNV GLYDYSAKSR SQGFALSLSG GADSAAVACL IRLMVELGVA
EVGIENFCAR LNLPQTELNT IEQLMPVLLV CVYQATRNSS ETTRNAAEKM AHAVGAEYLE
LDIENMVSGY RQHIEQAIGR SLNWTQDDLA LQNIQARARA PGVWMLANLR NALLLATSNR
SEAAVGYATM DGDTSGGLSP LAGIDKHFIR HWLRWLEQNA PNSPGVNPIP ALSYINTQQP
TAELRPGAEQ TDEADLMPYV LLNAIEKAAI RDRQTPWEIY CLLNSRFDYT AEQLLTWIER
FFQLWIRNQW KRERYAPSFH LDDESLDPKT WCRFPILSGG FSHELKEMRK NALSQMGEEP
G
//
