ID   A0A1H6RAX2_9GAMM        Unreviewed;       972 AA.
AC   A0A1H6RAX2;
DT   05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT   05-DEC-2018, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   RecName: Full=Protease 3 {ECO:0000256|ARBA:ARBA00017565};
DE            EC=3.4.24.55 {ECO:0000256|ARBA:ARBA00012449};
DE   AltName: Full=Pitrilysin {ECO:0000256|ARBA:ARBA00033450};
DE   AltName: Full=Protease III {ECO:0000256|ARBA:ARBA00031184};
DE   AltName: Full=Protease pi {ECO:0000256|ARBA:ARBA00029597};
GN   ORFNames=SAMN05421831_103226 {ECO:0000313|EMBL:SEI52998.1};
OS   Allopseudospirillum japonicum.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC   Oceanospirillaceae; Allopseudospirillum.
OX   NCBI_TaxID=64971 {ECO:0000313|EMBL:SEI52998.1, ECO:0000313|Proteomes:UP000242999};
RN   [1] {ECO:0000313|Proteomes:UP000242999}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 7165 {ECO:0000313|Proteomes:UP000242999};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endopeptidase that degrades small peptides of less than 7
CC       kDa, such as glucagon and insulin. {ECO:0000256|ARBA:ARBA00002184}.
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261}.
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DR   EMBL; FNYH01000003; SEI52998.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H6RAX2; -.
DR   STRING; 64971.SAMN05421831_103226; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000242999; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000242999};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          55..176
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          216..394
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          403..685
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          689..850
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   972 AA;  110094 MW;  093B34A48A9DA162 CRC64;
     MLSSRHYKAP IRLFSTLLFC VLSLIMSKLQ AATIHKPLND ARTYHAFTLE NGLQVLAITD
     PEAEKAAVAM HIGAGSHHEP KAYPRLAHFL EHMLFLGTRT YPQADAYAAY INQHGGQHNA
     MTAHEYTQYF FDVNHKDLEG ALARFAPFFS EPLLDPQYVQ REVKAVDSEY SAKLQQDGRR
     ILGALRQAFH PDHPLAHFSA GNAQTLPVDD PQLVPALREF FETYYRAPNM RLVVMGSQSV
     EQLTTWVRTY FAQIGANLDK KEESKPSDSL ALWSDQHPLP LRLEVQTLKD ERTLKLYFPV
     PHPKTLYPLR PVQFLANFLG HEGEGSLHAH LDALGWITQL GAGLDLPSGE QAFFALTLGL
     TPEGAQHQTD ILQKIFSYIQ LVRTQGMAEW RYQEMALLAQ QSFDFQEPLS GIHYVSRLAN
     NLYLYPVEDA LQASYRFGTF DVDAIQPYLE ALRPQNLLLL YAAPPQDFTE QTQYPWQKSP
     WYPTLYRLAP QTIQTLADIT DASTLGLQLP EANPYIADAL PILARASQEP MHTLLDTPEI
     KLWHSLSTLQ TPKANLFIYL KSPVSQRSLR EHMLTALWIN AASDAINTQA YPASLAGLTA
     HLYQASEGVT LRTLGYAPKQ IEFALDLLEQ VQTLQITPER FAQLQAEMQR ALSNMQHKPL
     TSQMFHALSS ALFDPAWSVQ EQAQALAQLT LADLQNFQTE FFTHLQLEIL SYGNLTTTEA
     QHLAEQLKSK LHLDASQVST PRRARLLPTQ TKTYLWKPEI QHNDHAQLLY LQAQQTDVQT
     QAQFALLAQM QRADFFHQLR TEQQLGYVVF TQEVSLLDRA GLVYVVQSPH IQPDALAQQI
     QNFLQAYQTQ MANLTDDALM RFKEGVIASL RQPYPRLSSR AATRWMDIRL GYIEQARKEA
     IADAVDQISL TQMHDFYQQL LTYPKLWLQV TGESATTVEN AANPEIAHPT LSAQVVDLQQ
     IRTWASYSPV LP
//