ID A0A1H6WXK4_9FIRM Unreviewed; 508 AA.
AC A0A1H6WXK4;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Sucrose-6-phosphate hydrolase {ECO:0000256|ARBA:ARBA00019623, ECO:0000256|RuleBase:RU362110};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758, ECO:0000256|RuleBase:RU362110};
DE AltName: Full=Invertase {ECO:0000256|ARBA:ARBA00033367, ECO:0000256|RuleBase:RU365015};
GN ORFNames=SAMN02910453_2299 {ECO:0000313|EMBL:SEJ17520.1};
OS Lachnospiraceae bacterium A10.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=1520821 {ECO:0000313|EMBL:SEJ17520.1, ECO:0000313|Proteomes:UP000198998};
RN [1] {ECO:0000313|EMBL:SEJ17520.1, ECO:0000313|Proteomes:UP000198998}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A10 {ECO:0000313|EMBL:SEJ17520.1,
RC ECO:0000313|Proteomes:UP000198998};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Enables the bacterium to metabolize sucrose as a sole carbon
CC source. {ECO:0000256|RuleBase:RU365015}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000256|RuleBase:RU362110};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC {ECO:0000256|ARBA:ARBA00004914, ECO:0000256|RuleBase:RU365015}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU365015}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR EMBL; FNYN01000007; SEJ17520.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H6WXK4; -.
DR OrthoDB; 9759709at2; -.
DR UniPathway; UPA00238; -.
DR Proteomes; UP000198998; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd08996; GH32_FFase; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR006232; Suc6P_hydrolase.
DR NCBIfam; TIGR01322; scrB_fam; 1.
DR PANTHER; PTHR43101; BETA-FRUCTOSIDASE; 1.
DR PANTHER; PTHR43101:SF1; BETA-FRUCTOSIDASE; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU365015};
KW Cytoplasm {ECO:0000256|RuleBase:RU365015};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Reference proteome {ECO:0000313|Proteomes:UP000198998}.
FT DOMAIN 29..339
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 342..496
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 508 AA; 58627 MW; 9F41740C289E8809 CRC64;
MNSQTLREAR KYEEAFEKLI PAEQRPKFHL SPRVGWMNDP NGFCIYKDKY HLFYQYHPYD
AHWGPMHWGH AESEDLLHWK FLPVALAPDE FYDKDGVFSG SAITLKDGRH LLIYTGVMKR
TVENGQMKEF QTQCVAIGDG LDYEKYENNP VINTDAIPEG ASKTDFRDPK IWEKADGTYR
CLVANRPADG SGQLLLFKSN DCIHWDFVKV FAENKNRYGL MWECPDFFTL DGKGVLLTSP
QDMLPEGFEF HNGNGNICII GSYDDDTETF TEESVQAVDY GIDFYAMQTI EMLDGRRVMI
GWMQNWDTTG AHDGSEPWFG QMSLPREISI RDGRLCQWPI KEVEDMRSNK VEYKEVVVEA
TRDLKASDSE LVKGGVSFEG IKGRCVDLEL EVKPKKFDEM FDQFVISIAA DEKFHTDIVL
RPKENIAKID RKFSGSRRAI VHQRRAHIPW KSESIKMRIV MDRYSMEVFF EDGKYVMTAS
LPTDVSCDGI YFNTNEDAVL NITKYDLN
//
