ID   A0A1H7FI16_9FIRM        Unreviewed;       293 AA.
AC   A0A1H7FI16;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   SubName: Full=Sortase A {ECO:0000313|EMBL:SEK22935.1};
GN   ORFNames=SAMN02910377_00392 {ECO:0000313|EMBL:SEK22935.1};
OS   Pseudobutyrivibrio ruminis.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC   Pseudobutyrivibrio.
OX   NCBI_TaxID=46206 {ECO:0000313|EMBL:SEK22935.1, ECO:0000313|Proteomes:UP000182321};
RN   [1] {ECO:0000313|Proteomes:UP000182321}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ACV-9 {ECO:0000313|Proteomes:UP000182321};
RA   Varghese N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; FNZX01000003; SEK22935.1; -; Genomic_DNA.
DR   RefSeq; WP_074788876.1; NZ_FNZX01000003.1.
DR   AlphaFoldDB; A0A1H7FI16; -.
DR   Proteomes; UP000182321; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   CDD; cd05827; Sortase_C; 1.
DR   Gene3D; 2.40.260.10; Sortase; 1.
DR   InterPro; IPR005754; Sortase.
DR   InterPro; IPR042002; Sortase_C.
DR   InterPro; IPR023365; Sortase_dom-sf.
DR   NCBIfam; NF033745; class_C_sortase; 1.
DR   NCBIfam; TIGR01076; sortase_fam; 1.
DR   Pfam; PF04203; Sortase; 1.
DR   SUPFAM; SSF63817; Sortase; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000182321};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        265..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   ACT_SITE        159
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605754-1"
FT   ACT_SITE        226
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605754-1"
SQ   SEQUENCE   293 AA;  32591 MW;  DBE0AB162E5F3C9D CRC64;
     MKQRVIKILL ILLFLVGFGL LTYPTISNEW NVYVQSTLIN NYEDSLSEMT EEDYTKEWEA
     ARAFNEQITE NAIGTDVFGA NADGDATTPT DEFLNSEYYS VLNVNGDGVM GYLSIPKINL
     KLSIMHGTFD EYIQTALGHM NGTALPIGGE GTHSVIVGHR GLPSAELFTN IDQLEVGDKF
     YIHVLDETLA YQVDMIYPMI EAEDITSLQN AMKVEEGKDY VTLFTCTPYG VNTHRLLVRG
     TRVEYLGEDE VPTGAAAVAE TVKNYYLIMG IAGFIAAVII ILIIRRIFKK KTA
//