ID A0A1H7Q9V2_STIAU Unreviewed; 861 AA.
AC A0A1H7Q9V2;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN ORFNames=SAMN05444354_10657 {ECO:0000313|EMBL:SEL44508.1};
OS Stigmatella aurantiaca.
OC Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
OC Archangiaceae; Stigmatella.
OX NCBI_TaxID=41 {ECO:0000313|EMBL:SEL44508.1, ECO:0000313|Proteomes:UP000182719};
RN [1] {ECO:0000313|Proteomes:UP000182719}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17044 {ECO:0000313|Proteomes:UP000182719};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
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DR EMBL; FOAP01000006; SEL44508.1; -; Genomic_DNA.
DR RefSeq; WP_075006770.1; NZ_FOAP01000006.1.
DR AlphaFoldDB; A0A1H7Q9V2; -.
DR OrthoDB; 9765468at2; -.
DR Proteomes; UP000182719; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR CDD; cd00367; PTS-HPr_like; 1.
DR Gene3D; 2.70.70.10; Glucose Permease (Domain IIA); 1.
DR Gene3D; 3.30.1340.10; HPr-like; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR000032; HPr-like.
DR InterPro; IPR035895; HPr-like_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR001020; PTS_HPr_His_P_site.
DR InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR00830; PTBA; 1.
DR NCBIfam; TIGR01003; PTS_HPr_family; 1.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR Pfam; PF00381; PTS-HPr; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR PRINTS; PR00107; PHOSPHOCPHPR.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SUPFAM; SSF51261; Duplicated hybrid motif; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55594; HPr-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
DR PROSITE; PS51350; PTS_HPR_DOM; 1.
DR PROSITE; PS00369; PTS_HPR_HIS; 1.
DR PROSITE; PS00589; PTS_HPR_SER; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:SEL44508.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000182719};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:SEL44508.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 36..140
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000259|PROSITE:PS51093"
FT DOMAIN 177..266
FT /note="HPr"
FT /evidence="ECO:0000259|PROSITE:PS51350"
SQ SEQUENCE 861 AA; 89852 MW; 68598F0CC4976AAD CRC64;
MSTLKPAGAA ASQTLSKLQL AAPIAGWATV LEEVPDPAFA QRMVGDGIAV DPTSAELRSP
CDGVVLSVHA SRHACTLRTL TGAEILLHIG IDTVSLRGEG FTPHVSEGQS VRTGEPLISF
DMDLLARKAR SLMTVMVVAN GDAYTVTHRV QNRSVATGEP LLEISGGELA AQAETGTETA
ECRVRLLIPH GLHARPAAAF VRHARPYPGT IHVGLKGRSA NGKSVVALMG LGAHHGDTLT
LSVRGGRAAQ VAQELAEQVT LGLGDAVRPL AEPSAAPAEK APGVAVAPAQ PFAPGTEVLL
KGTLAAAGLA VGQAVRVNEE EPALSQQGRG PQEEERRLAE ALAGVRRDIE ALLQTEGKGS
AARTEIFQAH LALLDDPEFT GAAGQGIAIG QSAEWAWKAA IEKHVQVLHS LADPLLQERM
GDLRDIGRRV IASLTGQDGA RVPANLPPGA ILVANELLPS DLAAVPPGRL AALCTARGGP
TSHVAILAAG MGIPAVVALG EGVLRVPPGA PLIVNGDRGE VHVHPPEAAR ESTQNTLRAR
AARRKTDLAT AHEDCRTSDG VRIEVFANLG RPGDAASAAA QGAEGCGLLR SEFLFLERLT
APSEDEQAAQ YQESATGLQN RPLIIRTLDV GGDKPLAYLP LPAEENPVLG LRGVRVSLRY
PELLRTQVRA ILRVKPEGVC RILVPMITSA HELQAVRAVV EQERRALGYA APVLVGAMVE
VPVAAVLADR LAVHADFLSI GTNDLTQYAL AMDRGNAYLA PQLDSLHPGV LRLVAQTVEG
ASKHGRPVAV CGGIASDPQA APLLIGLGVT ELSATPAVIP GLKAFIRTLS LAQCQEAARA
ALELTNGDEV RALVARTWQS Q
//
