ID   A0A1H7TLR8_9GAMM        Unreviewed;       915 AA.
AC   A0A1H7TLR8;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE            EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE   AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE   AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN   ORFNames=SAMN05428989_2702 {ECO:0000313|EMBL:SEL85721.1};
OS   Pseudoxanthomonas sp. GM95.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Pseudoxanthomonas.
OX   NCBI_TaxID=1881043 {ECO:0000313|EMBL:SEL85721.1, ECO:0000313|Proteomes:UP000199164};
RN   [1] {ECO:0000313|Proteomes:UP000199164}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GM95 {ECO:0000313|Proteomes:UP000199164};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC         amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC         of amylopectin and glycogen.; EC=3.2.1.41;
CC         Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR   EMBL; FOAX01000002; SEL85721.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H7TLR8; -.
DR   STRING; 1881043.SAMN05428989_2702; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000199164; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR   CDD; cd02860; E_set_Pullulanase; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR024561; Pullul_strch_C.
DR   InterPro; IPR040671; Pullulanase_N2.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF11852; Pullul_strch_C; 1.
DR   Pfam; PF17967; Pullulanase_N2; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 2.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           25..915
FT                   /note="pullulanase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5011565173"
FT   DOMAIN          387..728
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   915 AA;  98765 MW;  83625C0907869B9B CRC64;
     MGVRATARLA GLLLAVHLAA PARADARSDC DGPAFARVLQ PSTQPAIDKT ASAYWLDAAH
     LRWPGHPQEA RYALFVADAP TLKLEGDRLL GADRRIALQR FDAPLTDDLT QRFGFIGAGA
     TLALPPAIQV ELDTLLQGQV VLAQLDANGR VIDHTALQLP GALDDRYAAA AQVPLGATLT
     ANATQFALWA PTARAVQLCL YHDDASDAFE MQPMTRDAAT GVWQRHFAGR RGGTFYRFLV
     DVFVPGTGLV RNRVSDPYAV SLGVNGERSV VLDLDDPALA SPGWRQQSRP PAADDNSQLS
     IYELHVRDFS RDDASVPAAH RGKYLAFTDT SSDGMHHLAA LAQAGLTDVH LLPAFDFASV
     PEAGCVTPQI PQTPTDSQLQ QAAVMASAAR DCFNWGYDPL HFGAPEGSYA SDAADGTRRV
     IEFRQMVMAL HALGLRVGMD VVYNHTSASG QQASSVLDRI VPGYYQRLDA QGRVATSTCC
     ANTATEHRMM ARLMIDTAVR WARDYRVDGF RFDLMGHQPR AAMEALQRAV DAAAGRHIPL
     LGEGWNFGEI VDGARFVQAA QGRLGGTGIG TFSDRARDAI RGGTPGDRGD ALRDNVGWLN
     ADWRRDADSP ALAHAADLLR LGLAGTLVDF PVPTHDARTV RGDALDYRGQ PAGYATAPGE
     VVNYVENHDN HTLFDIDALR APDGTTAMDL ARMQALGLAT VAFSQGAAYF HAGGERLRSK
     SLDRNSFDSG DAFNRLTWTG ADNGFVHGLP PAPDNQADWP LLAPKLADPN LKPGAAEIAF
     ASARFEDWLR IRASSSLFRL RTADEVRARL RFPALSGDTA ACVVVGHLHG GELDGQRIGN
     DGFAEVLYLF NACPDARAPV VADARGKPYV LHPVQRETRP RADPQLAQAR FDRTTGRFTL
     PGRSAVVYVI ERARQ
//