ID A0A1H8BEJ6_9BACL Unreviewed; 535 AA.
AC A0A1H8BEJ6;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=SAMN05444955_102148 {ECO:0000313|EMBL:SEM81361.1};
OS Lihuaxuella thermophila.
OC Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
OC Lihuaxuella.
OX NCBI_TaxID=1173111 {ECO:0000313|EMBL:SEM81361.1, ECO:0000313|Proteomes:UP000199695};
RN [1] {ECO:0000313|EMBL:SEM81361.1, ECO:0000313|Proteomes:UP000199695}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 46701 {ECO:0000313|EMBL:SEM81361.1,
RC ECO:0000313|Proteomes:UP000199695};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947,
CC ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; FOCQ01000002; SEM81361.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H8BEJ6; -.
DR STRING; 1173111.SAMN05444955_102148; -.
DR OrthoDB; 291295at2; -.
DR Proteomes; UP000199695; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.40; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR025711; PepSY.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF3; NEUTRAL PROTEASE B; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF03413; PepSY; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000199695};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..28
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 29..535
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023155442"
FT DOMAIN 81..130
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 145..218
FT /note="PepSY"
FT /evidence="ECO:0000259|Pfam:PF03413"
FT DOMAIN 226..370
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 373..534
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 363
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 450
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 535 AA; 59164 MW; 5D4EA596B3347F61 CRC64;
MKKRAAVGMV LSIALGASSF LFQQPAEAKG GEEIRYSKEY KTPAYIGEKW SKPKNLKNEE
IVWAYLHAKK SLFKLKGDVK SQFKILKQEK DSLGMTHYRV QEVYHGIPVY GSDQTVHTDR
DGNVTSFFGT VIPDLGAKKI QTTAKIGKEQ AVQIVRKDLE AQPGNISRLA SDPDTGLYIY
PHQGNYYLAY HVKVSVIQPE PGYWHYFINA VNGEVIRKYN TVHQLTGSGR GVLGDRKTFE
VDHKYGKYYL KGTSRGGGIK TYNAYHVIYE SQLPGTMVSS KTNYFLDGAA VDAHAYAEKV
YDYFKNVHGR NSYDGNGAPV ISSVHVGRNW NNAAWIGTQM VYGDGDGIVF RPLSGALDVV
GHELTHAITE KTANLEYHNE SGALNESISD IFGAMVDSGD WLLGEDVYTP KQSGDAFRSM
SNPTQYDQPD HYSRYVRLPD TPDGDYGGVH INSGINNKAA YLLAEGGTHY GVNVTGIGRA
KTAKIYYRAL TLYLTSRSTF ADMRQAAIKA ATDLYGSASQ EVQSVKNAYS AVGVY
//
