ID A0A1H8EN55_9BACI Unreviewed; 920 AA.
AC A0A1H8EN55;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=SAMN05192533_11022 {ECO:0000313|EMBL:SEN20228.1};
OS Mesobacillus persicus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Mesobacillus.
OX NCBI_TaxID=930146 {ECO:0000313|EMBL:SEN20228.1, ECO:0000313|Proteomes:UP000198553};
RN [1] {ECO:0000313|Proteomes:UP000198553}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B48,IBRC-M 10115,DSM 25386,CECT 8001
RC {ECO:0000313|Proteomes:UP000198553};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR EMBL; FOBW01000010; SEN20228.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H8EN55; -.
DR STRING; 930146.SAMN05192533_11022; -.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000198553; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Pyruvate {ECO:0000313|EMBL:SEN20228.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000198553}.
FT ACT_SITE 150
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
FT ACT_SITE 578
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ SEQUENCE 920 AA; 106085 MW; 494FD8CE927CBC83 CRC64;
MTTGLMVNDS SLPLRRDVKM LGAMLGEILV YHGGNELLDK VEKIRIMCKD LRSEYKTEVY
EKLKQEIAKL DGPMRKHVIR AFAVYFHLIN IAEQNHRIRR RREYQLQTDT NAQPGSIEHA
VQSLKDNQIS SDLIQNVLNK ISLELVITAH PTEATKRSIL ELQKRIADIL KTLDNPLLSK
KERKKLKESL FNEMMALWQT DEIRHRKPTV IDEVRNGLYY FDQTLFDVLP EIHQEVEESL
EEHYPDQEWN VPNFLRFGSW IGGDRDGNPH VTPDVTWETL NKQKRLVLKK YKNVLVELMK
RFSHSTARVQ VSTELLESLP SDEAAYLKED RRWPIVNEVY RRKFAVIIQR LKEVGESEVG
YKSSEELLDD LRMIKRSVYQ HHPTNHELKK LQKLIRQVQL FGFHLATLDI RNHSGEHESA
ITEILRKARV ADNYQNLSEE EKVKVLQSIL EDPRSLLLLH EDYSKETQQM LDVFTLIKKA
HKEFGKRSIS VYLISMTQSP SDILEVLVLA KEAGIYRLHA DGSVESDLNV APLLETIDDL
TAGRNIMETL FQMPLYREHL LALNNQQEIM LGYSDGSKDG GTLTANWKLY KAQVEIQEMA
KNYQIGLKFF HGRGGSLGRG GGPLHRSLMS QPLETLGEGI KITEQGEVLS SRYLIEDIAY
RSLEQATSTL LRAAAHVLNE SEQAHHRDAR WVEAIEKMTE VSLRKYQDLV FADPDFITYF
NEATPLHEIG ELNIGSRPMS RKNSAQFENL RAIPWVFAWT QSRQLLPGWY AAGSGLESFA
GQSEDNLKLL QEMYQEWPFF RSTVDNLQMA LMKADLTTAK EYSELVTDKN VAERIFSNIV
EEYNRTKEIL LKIVEDEELL DHTPNIKESV HRRNPYVDPL NFIQVELIKE IRDAQEPDEE
LVTQVLLTIS GVAAGLRNTG
//
