ID A0A1H8KEE3_9RHOB Unreviewed; 940 AA.
AC A0A1H8KEE3;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=SAMN04488103_10959 {ECO:0000313|EMBL:SEN91225.1};
OS Gemmobacter aquatilis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae; Gemmobacter.
OX NCBI_TaxID=933059 {ECO:0000313|EMBL:SEN91225.1, ECO:0000313|Proteomes:UP000198761};
RN [1] {ECO:0000313|EMBL:SEN91225.1, ECO:0000313|Proteomes:UP000198761}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 3857 {ECO:0000313|EMBL:SEN91225.1,
RC ECO:0000313|Proteomes:UP000198761};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; FOCE01000009; SEN91225.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H8KEE3; -.
DR STRING; 933059.SAMN04488103_10959; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000198761; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022839};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000198761};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 9..272
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 327..522
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 697..900
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 940 AA; 102051 MW; 36F44B109FA3903E CRC64;
MTGDQQGFGK GCHLHLIDGS AFIFRAYHAL PPLTRKSDGL PIGAVAGFCN MLFRYVEGNK
GPDAPTHVAV IFDHSSKTFR NEIYPAYKAN RPELPEDLRP QFPLTRDATR AFNIACIETE
GYEADDIIAT LSCKARDAGG RVTIISSDKD LMQLIGDGVV MFDAMKNKTI DRDEVFEKFG
VAPERVVDVQ ALAGDSVDNV PGAPGIGIKT AALLITEYGD LETLLDRAGE IKQPKRREAL
VNNADLIRVS KRLVQLDCET PLDFSLESLE VLAPEPEVLL PFLTEMEFRT LTKRVADSLK
VAAPVAQAAP EAEVAAPEVL PFEAAGYEAV TTAEALAEWV ARIRARGYVA FDTETTSLDE
MRADLVGISL AVEAGRACYI PIGHRAGGGD LFASDSLVEG QLPLAEVLAA LKPVLEDPSI
LKIGQNMKYD AKIMARLGVT VAPIDDTMLM SYAMHGGLHN HGMDELSERY LNHRPIPIKD
LIGSGKSAVT FDRVAVADAV KYAAEDADVT LRLWQLFKPR LHRAQVTTVY ETLERPLIPV
LAEMEMHGIQ VDRDTLSRMS NAFAQKMAGL EAEIHELAGG PFNVGSPKQL GEILFDRLGV
PGGVKGKTGA YATGADVLED ITTLEDSQPQ GARLAARLLD WRQLSKLKST YTDALQDHIN
PETGRVHTSY SIAGANTGRL ASTDPNLQNI PVRSEEGRRI REAFVAPEGR VLVSLDYSQI
ELRILAHVAG IPALKQAFEQ GHDIHAMTAS EMFNVPLDQM TPEIRRQAKA INFGVIYGIS
GFGLARNLRI PRGEAQGFID RYFERFPGIR AYMDDTIAFA KAHGYVQTLF GRKIHTPEIN
AKGPGAGFAK RAAINAPIQG TAADVIRRAM IRMPQAIAGL DAKMLLQVHD ELLFEVAEAD
AAALTQAAKE VMEAAAAPAV HLSVPLTVEA GQGQSWSAAH
//
