ID A0A1H8XUH2_9SPHI Unreviewed; 715 AA.
AC A0A1H8XUH2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=SAMN05428947_11843 {ECO:0000313|EMBL:SEP43486.1};
OS Mucilaginibacter sp. OK283.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=1881049 {ECO:0000313|EMBL:SEP43486.1, ECO:0000313|Proteomes:UP000199509};
RN [1] {ECO:0000313|EMBL:SEP43486.1, ECO:0000313|Proteomes:UP000199509}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OK283 {ECO:0000313|EMBL:SEP43486.1,
RC ECO:0000313|Proteomes:UP000199509};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; FODR01000018; SEP43486.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H8XUH2; -.
DR STRING; 1881049.SAMN05428947_11843; -.
DR Proteomes; UP000199509; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013739; Beta_galactosidase_C.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08533; Glyco_hydro_42C; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084}.
FT DOMAIN 60..437
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 456..658
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 675..714
FT /note="Beta-galactosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08533"
FT ACT_SITE 195
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 359
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 156
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 194
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 367
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 715 AA; 80860 MW; 1031063FFE930A33 CRC64;
MKFMVKQLKT AAIEKPGTFL MRKLITTIIL GILIPSAALK AQEAGRFFPE KDLVTTGIYY
YPEHWSEAQW ERDIKKISDM GFEFVHLAEF AWFKMEHEEG RFDFTWLDRV VGLCTKYHLK
VLLCTPSATT PTWMRINYPE TFVMDGHYIR AENGTRGLES MVNPKFRGFV QRIVAEMAKR
YGQNKNVMGW QVDNEPGAVP DYSPSSQDAF RTWLKNKYKT IDALNTAWGA AFWSQWFNSF
DQVIIPNTNL VGWWGNNPHA LLDFKRYSAD AQAEFLDFQA GVLRSYVSKT QFVTTNYTAV
CTGSDPNRTA KLDFATYTAY PNGGTDNLGE SGFRLGNSDV VLFASEYYKS VGGVSGVLEI
QPGPVNWGSY NPLLLPGTVR LWLYHSFAAG GKVACSYRFR QILYGAEQYH AGVIQTDGVT
PSQGGKDYMQ FMKEITELRK QFTPGAKMPA KLTARSTALL WNVENYWSID RQRQTGQWNT
WDYPVKFLKT ARSLGAPVDV IQETADLSKY KCVIVPAYEM IDSALVKKWN DYVAKGGHLV
ITCRTGTKDR RGHFWEGETA LPISNLIGAH ISQTDMLSSY GKGDILMNSN HYPWHSWADL
LVPNDTAGVL ATYNNQFYKG KAAVVKHRIG KGDVTYIGVD TDDAKLEKDL LRQIYKDAGA
TTEDYPPGVF VYWRDGFYTA LNYSSENYTV NLPDNAKVLI GEKTLKPSGV LVWTE
//
