ID   A0A1H8YQC4_9PSEU        Unreviewed;       851 AA.
AC   A0A1H8YQC4;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   SubName: Full=Lysyl-tRNA synthetase, class II {ECO:0000313|EMBL:SEP54404.1};
DE   Flags: Fragment;
GN   ORFNames=SAMN04489732_1461 {ECO:0000313|EMBL:SEP54404.1};
OS   Amycolatopsis saalfeldensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Amycolatopsis.
OX   NCBI_TaxID=394193 {ECO:0000313|EMBL:SEP54404.1, ECO:0000313|Proteomes:UP000198582};
RN   [1] {ECO:0000313|EMBL:SEP54404.1, ECO:0000313|Proteomes:UP000198582}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 44993 {ECO:0000313|EMBL:SEP54404.1,
RC   ECO:0000313|Proteomes:UP000198582};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; FOEF01000046; SEP54404.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H8YQC4; -.
DR   STRING; 394193.SAMN04489732_1461; -.
DR   OrthoDB; 9801152at2; -.
DR   Proteomes; UP000198582; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd04322; LysRS_N; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR024320; LPG_synthase_C.
DR   InterPro; IPR044136; Lys-tRNA-ligase_II_N.
DR   InterPro; IPR018149; Lys-tRNA-synth_II_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR031553; tRNA-synt_2_TM.
DR   PANTHER; PTHR34697; PHOSPHATIDYLGLYCEROL LYSYLTRANSFERASE; 1.
DR   PANTHER; PTHR34697:SF2; PHOSPHATIDYLGLYCEROL LYSYLTRANSFERASE; 1.
DR   Pfam; PF09924; LPG_synthase_C; 1.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   Pfam; PF16995; tRNA-synt_2_TM; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PRINTS; PR00982; TRNASYNTHLYS.
DR   SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   4: Predicted;
KW   Aminoacyl-tRNA synthetase {ECO:0000313|EMBL:SEP54404.1};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Ligase {ECO:0000313|EMBL:SEP54404.1};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198582};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        21..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        52..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        86..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        130..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        160..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          755..851
FT                   /note="Aminoacyl-transfer RNA synthetases class-II family
FT                   profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50862"
FT   NON_TER         851
FT                   /evidence="ECO:0000313|EMBL:SEP54404.1"
SQ   SEQUENCE   851 AA;  93718 MW;  5D3DAC4BEC84EB58 CRC64;
     MGTPASARPR IPGWKARAGG VTATVVQLAA VFSVVLLIAG GTHGHLLNAI DMAFSALSVP
     TSASLVIVLL LVVLGGALRR RKKAALYTLL LFQVAGLLLT LAMQAVLLWA PSLLTLGPRQ
     IRHIPMQVSV LTIADVISVL LILFLLTLRP AFPARLAPGA WRNGLSMLLG GLLVVIVLGW
     GLSEAFPGHL GDTWERFAWV VNHATGENIQ LRRIGVGEGP AWLDVFLDLG ATFVATAALY
     MLFRGVRSRR LRTDDEELRL RRLLAEFGED DSLGYFATRR DKSVVFAPNG RAAVTYRVLG
     GTSVASADPI GDPEAWPDAV RAWLDETRSY GWTPGALGAS ERGAKVYAAA GLKALEFGDE
     AVLDIREFSL TGPERKSVRQ AVKRIERAGY TSRVRRHSEI PDDEMAELLR HAQQWRGAET
     ERGFSMALGR LGDPSDGRSV MVEAYDAEGR LRGMLSFVPW GRRGLSLDLM RRDRDAENGL
     NEFMVAEVVA AGQQLGAQRI SLNFAMFRAV FSEGERIGAG PVLRAWRGVL GVASRFFQLE
     SLYRSNAKYG PDWEPRFLCY SSARRLPRVG IVAGALEGFV PTGRSRSLRL ENVGQEFVAE
     AKRIDESSAK AVPKAARRPE QVRVRVAKLD KLRDLGIDPY PVGFRREDLL GDIVRKYADL
     GPDSRTGHRV RVAGRVLALR TLGGLCFARI KDFSGELQLM LDARELDLTG WRGGVDLGDH
     VGVSGRVVTS RRGELSVLVD EWTVTAKCLH PLPDKRKGLT DPETRVRQRY LDLAVNPESA
     QMLRFRSTVV RAVRERLHQG DYLEVETPML QTVHGGANAR PFVTHINAYD MRMYLRIAPE
     LYLKRLCVAG V
//