ID A0A1H8YXE7_9GAMM Unreviewed; 858 AA.
AC A0A1H8YXE7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=CRISPR-associated endonuclease/helicase Cas3 {ECO:0000313|EMBL:SEP56900.1};
GN ORFNames=SAMN05421693_10153 {ECO:0000313|EMBL:SEP56900.1};
OS Ectothiorhodospira magna.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Ectothiorhodospira.
OX NCBI_TaxID=867345 {ECO:0000313|EMBL:SEP56900.1, ECO:0000313|Proteomes:UP000199496};
RN [1] {ECO:0000313|EMBL:SEP56900.1, ECO:0000313|Proteomes:UP000199496}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B7-7 {ECO:0000313|EMBL:SEP56900.1,
RC ECO:0000313|Proteomes:UP000199496};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
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DR EMBL; FOFO01000001; SEP56900.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H8YXE7; -.
DR STRING; 867345.SAMN05421693_10153; -.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000199496; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR PANTHER; PTHR47959; ATP-DEPENDENT RNA HELICASE RHLE-RELATED; 1.
DR PANTHER; PTHR47959:SF16; CRISPR-ASSOCIATED NUCLEASE_HELICASE CAS3-RELATED; 1.
DR Pfam; PF18019; Cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Endonuclease {ECO:0000313|EMBL:SEP56900.1};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:SEP56900.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000199496}.
FT DOMAIN 17..207
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 529..704
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 858 AA; 96529 MW; 507E37867EE3677B CRC64;
MEAIYKYWGK ARAEADAQDF CHLLPYHCLD VTAVADAWWQ SSPVIQRLMV VHGDADPVKI
HAWVLFFVAL HDLGKFDIRF QLKSIPALLH LRPDFDLDSV EPEPYAHGPA GCGWLEHAVP
LHSDDRYDCW QPWMQAVAGH HGDLSSPKIP ADPDADEEVC RFDKEARRAW LQSLARLFLE
PAGLSLLDDP PTCPQIMAGF CSIADWIGSN TDWFEYRDIV QPLQEYWEAE AKKRALFALE
STGMLGKGPA QGGMAAIFPD CSPRQIQCLV DDLPREAGLL LVEGPTGCGK TEFSLAYAAH
LLATGVAEAI IFALPTQATA NAMLNRLEKV APNLFPGQKN VVLAHGRASR HEGYMALKEA
GRRLTAQGEE DAAVQCADWL AQSRKRVFLG QIGVCTIDQV LISVLAIRHH FVRAFGVRRA
VLIVDEVHAY DAYMYGLLTR VLHAQATAGG SAILMSATLP AYQRDLLLDA WGGEVPEQQS
SAYPLISWAG RGTPVRYWDL KGEPAHLPES RTVFLERRER VDLRLSDQDI DQVIQAANEG
ACVAVICNLV SEAQGLWRRL RERSSQVPVD LFHARYLFAD RQVIEQRVME YYGRDRMATG
RILVATQVVE QSLDLDFDWL ITQLCPVDLL FQRMGRLHRH PRTDRPAGFE DPRCTVLLPP
DEQYGLHEVI YQCRRVLWRT HRRLQETDRI TFPDVYRPWI ESVYAEEAWL DEPEAVLQNW
ETFQNSALGK FFGARQLAEA DMMPLQDTDA NAAAMTRDGD NGLKVLLLQS MPQGPAYLNG
RQESALDDFE RREAEDLNAV NAPKGWKAAL GNPDEHGRYR LILEPQADGS WSVGAMRYSR
EIGLEWREGD SSLPSQPQ
//
