ID A0A1H8YXS2_9ACTN Unreviewed; 750 AA.
AC A0A1H8YXS2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=ATP-dependent RecD-like DNA helicase {ECO:0000256|HAMAP-Rule:MF_01488};
DE EC=3.6.4.12 {ECO:0000256|HAMAP-Rule:MF_01488};
GN Name=recD2 {ECO:0000256|HAMAP-Rule:MF_01488};
GN ORFNames=SAMN05216481_101162 {ECO:0000313|EMBL:SEP57020.1};
OS Streptomyces radiopugnans.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=403935 {ECO:0000313|EMBL:SEP57020.1, ECO:0000313|Proteomes:UP000199055};
RN [1] {ECO:0000313|EMBL:SEP57020.1, ECO:0000313|Proteomes:UP000199055}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 4.3519 {ECO:0000313|EMBL:SEP57020.1,
RC ECO:0000313|Proteomes:UP000199055};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent ATPase and ATP-dependent 5'-3' DNA helicase.
CC Has no activity on blunt DNA or DNA with 3'-overhangs, requires at
CC least 10 bases of 5'-ssDNA for helicase activity. {ECO:0000256|HAMAP-
CC Rule:MF_01488}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01488};
CC -!- SIMILARITY: Belongs to the RecD family. RecD-like subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_01488}.
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DR EMBL; FOET01000001; SEP57020.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H8YXS2; -.
DR STRING; 403935.SAMN05216481_101162; -.
DR Proteomes; UP000199055; Unassembled WGS sequence.
DR GO; GO:0043139; F:5'-3' DNA helicase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR CDD; cd17933; DEXSc_RecD-like; 1.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 1.10.10.2220; -; 1.
DR Gene3D; 2.30.30.940; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_01488; RecD_like; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR006345; DNA_helicase_RecD-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR029493; RecD-like_HHH.
DR InterPro; IPR041451; RecD-like_SH13.
DR InterPro; IPR010994; RuvA_2-like.
DR InterPro; IPR027785; UvrD-like_helicase_C.
DR NCBIfam; TIGR01448; recD_rel; 1.
DR PANTHER; PTHR43788; DNA2/NAM7 HELICASE FAMILY MEMBER; 1.
DR PANTHER; PTHR43788:SF6; RECBCD ENZYME SUBUNIT RECD; 1.
DR Pfam; PF13245; AAA_19; 1.
DR Pfam; PF14490; HHH_4; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF18335; SH3_13; 1.
DR Pfam; PF13538; UvrD_C_2; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR SUPFAM; SSF47781; RuvA domain 2-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_01488}; DNA-binding {ECO:0000256|HAMAP-Rule:MF_01488};
KW Helicase {ECO:0000256|HAMAP-Rule:MF_01488};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_01488};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_01488}; Reference proteome {ECO:0000313|Proteomes:UP000199055}.
FT DOMAIN 360..557
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT BINDING 371..375
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01488"
SQ SEQUENCE 750 AA; 81233 MW; 7B1EFA53EC84C3C2 CRC64;
MSVPGVTVCD MSFQPAVLEA VLERITYANE ENGYTVARVD TGRGGELLTV VGSLLGAQVG
ESLRMEGRWG SHPQYGRQFT VENYTTVLPA TVQGIRRYLG SGLIKGIGPK IAERIVDHFG
LETLEVIENE PERLIEVPGL GPKRTKLIGA AWEEQKAIKE VMVFLQGVGV STSIAVRIYK
KYGDASISVV RNQPYRLAAD VWGIGFLTAD RIAQAVGIPH DSPDRVKAGL QYALSQSADQ
GHCYLPEERL IADTVKLLQV DTGLVIDCLG ELAGEEEGVV REQVPDPEHP GQRVSAVYLV
PFHRAELSLS AQLLRLLRTE EDRIPAFRDV DWEAALSWLA GRTGAELAPE QRDAVKLALT
QKVAVLTGGP GCGKSFTVRS VVELARAKRA KVVLAAPTGR AAKRLSELTG AEASTVHRLL
ELRPGGDAAY DRERPLDADL VVVDEASMLD LLLANKLIKA VAPGAHLLLV GDVDQLPSVG
AGEVLRDLLA EGSPVPAVRL TRIFRQAQQS GVVTNAHRIN SGVPPITQGL PDFFLFVEDD
TEEAGRLTVD VAARRIPAKF GLDPRRDVQV LAPMHRGPAG AGTLNGLLQQ AITPGRPDLP
EKRFGGRVFR VGDKVTQIRN NYEKGANGVF NGTVGVVTSL STEEQRLTVR TDEDEEVGYD
FDELDELAHA YAVTIHRSQG SEYPAVVVPV TTGAWMMLQR NLLYTAVTRA KRLVVLVGSR
RALGQAVRTV SAGRRCTALD HRLRGTPHPA
//
