ID A0A1H9F8Q0_9BACT Unreviewed; 759 AA.
AC A0A1H9F8Q0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Citrate hydro-lyase {ECO:0000256|ARBA:ARBA00029682};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=SAMN05444359_108130 {ECO:0000313|EMBL:SEQ34259.1};
OS Neolewinella agarilytica.
OC Bacteria; Bacteroidota; Saprospiria; Saprospirales; Lewinellaceae;
OC Neolewinella.
OX NCBI_TaxID=478744 {ECO:0000313|EMBL:SEQ34259.1, ECO:0000313|Proteomes:UP000199021};
RN [1] {ECO:0000313|Proteomes:UP000199021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 24740 {ECO:0000313|Proteomes:UP000199021};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; FOFB01000008; SEQ34259.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1H9F8Q0; -.
DR STRING; 478744.SAMN05444359_108130; -.
DR InParanoid; A0A1H9F8Q0; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000199021; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015932; Aconitase_dom2.
DR InterPro; IPR006248; Aconitase_mito-like.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01340; aconitase_mito; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000199021}.
FT DOMAIN 34..479
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 558..686
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 759 AA; 82218 MW; B1CC44762594B711 CRC64;
MAFDIDLIKK VYADLPGKVA EARKVLGRPL TLSEKILYSH LHEESPLANY GRGKDYVMFA
PDRVAMQDAT AQMALLQFMM AGRDKVAVPS TVHCDHLIQA KIGADADLQH AINTSNEVFN
FLESVSDKYG IGFWKPGAGI IHQVVLENYA FPGGMMIGTD SHTVNAGGLG MVAIGVGGAD
AVDVMAGMAW ELKNPKLIGV KLTGKLSGWS APKDVILKVA DILTVKGGTG AIVEYFGEGA
KSMSCTGKGT ICNMGAEIGA TTSTFGYDES MDRYLRATGR EDVADAAKEV AEHLTADAEC
YADPEKYFDQ VIEINLSELK PLINGPFTPD LSTAAGSEMT ERATANDWPL DVEWGLIGSC
TNSSYEDLSR AASIARQAVA KGLTTKAEFG INPGSEQVRF TTERDGIIKA FEDLNAKIFT
NACGPCIGQW ARYNDPMNAP KNSIIHSFNR NFSKRADGNP NTHAFVASPE MVAAIAISGR
LDFNPMTDTL VNDKGEEVML DEPTGYELPP DGFDVEDRGF NAPAEDGSGV EVVVAPTSDR
LQLLTPFVPI TQDDMTDMRV LIKVKGKCTT DHISMAGPWL KYRGHLENIS ENCYTGAINF
FNDKANKVLN YVVNDYVAVP DSAKAYQAKG IGTVVFGEEN LGEGSSREHA AMEPRFLGVN
AVIVKSFARI HETNLKKQGM LALTFVDKDD YDKVRQDDVV TIKGFDQMAP GKNLTIKLDH
HDGTNDEFEV AHTYNAAQIE WVRAGSALNK IRQDVAAGA
//
