ID   A0A1H9G906_9BACI        Unreviewed;       458 AA.
AC   A0A1H9G906;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=23S rRNA m(5)U-1939 methyltransferase {ECO:0000313|EMBL:SEQ46582.1};
GN   ORFNames=SAMN05216362_11460 {ECO:0000313|EMBL:SEQ46582.1};
OS   Piscibacillus halophilus.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Piscibacillus.
OX   NCBI_TaxID=571933 {ECO:0000313|EMBL:SEQ46582.1, ECO:0000313|Proteomes:UP000199427};
RN   [1] {ECO:0000313|EMBL:SEQ46582.1, ECO:0000313|Proteomes:UP000199427}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21633 {ECO:0000313|EMBL:SEQ46582.1,
RC   ECO:0000313|Proteomes:UP000199427};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; FOES01000014; SEQ46582.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1H9G906; -.
DR   STRING; 571933.SAMN05216362_11460; -.
DR   OrthoDB; 9804590at2; -.
DR   Proteomes; UP000199427; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   NCBIfam; TIGR00479; rumA; 1.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000199427};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          7..65
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        414
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        414
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         289
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         318
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         339
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         387
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   458 AA;  51887 MW;  9C76DC05B21C251E CRC64;
     MAKKIAPVKK NEYIDLEFVD LTHEGNGVGK IDGYPVFVPY GLPGEKAKVK IVKVKKNVAF
     GKLIELIEPS ENRIEPPCDV FYQCGGCQIQ HMSYERQLKM KQKQVQDVMH KIAHMPDVPV
     HPVIGMDEPW SYRNKVQIPV GERDGKVLTG FYQKRSHRII DMDTCPVQNE ANDHIVREMR
     GIIEELGIEP YDEETHRGVI RHIVVRSGYH TGEIMVVLVT RTKKIPNQEA LIEKIKELNP
     NIKGIIHNVN SKQTNVIMGR ESKTLWGEDV IIDKIGDLEF AISAHSFFQV NPEQTERLYE
     QALKFANLTG NETVIDAYCG IGSISLFLAQ HAKKVYGVEV VPQAVDDAKD NAKRNHIDHA
     EFFVGEAEKV MPWWRAQGLR PNVIVVDPPR KGCDEELLKA MIDMKPERIV YVSCNPSTLA
     RDIKILADGG YSAKQIQPVD MFPQSNHIES VTELVLEN
//