ID A0A1I0E319_9FIRM Unreviewed; 641 AA.
AC A0A1I0E319;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=SAMN04489758_10915 {ECO:0000313|EMBL:SET39026.1};
OS Thomasclavelia cocleata.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Coprobacillaceae; Thomasclavelia.
OX NCBI_TaxID=69824 {ECO:0000313|EMBL:SET39026.1, ECO:0000313|Proteomes:UP000198558};
RN [1] {ECO:0000313|Proteomes:UP000198558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1551 {ECO:0000313|Proteomes:UP000198558};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
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DR EMBL; FOIN01000009; SET39026.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I0E319; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000198558; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008652; P:amino acid biosynthetic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR006250; Aconitase_put.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR006251; Homoacnase/IPMdehydase_lsu.
DR NCBIfam; TIGR01342; acon_putative; 1.
DR NCBIfam; TIGR01343; hacA_fam; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 2.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000198558}.
FT DOMAIN 9..283
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 283..407
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 521..572
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 641 AA; 69619 MW; C3072572492151E1 CRC64;
MGMNLAYKIL SSKLKDGELI PGQQIGIQID QTLTQDSTGT MAYLQLEAMN IKHVAVEKAV
AYIDHNMLQT GFENMDDHEF IRSVAKKHGI VFSKPGNGVC HQLQLENFSK PGKTLVGSDS
HTPTCGAMGM IAIGAGGLDV AVAMATGKYY LQCPSVVKVN LTGKKAPWVS AKDIILYILQ
QLTVKGGVNK IIEYTGEGIA SLSLTDRATI CNMGAELGAT TSVFPTDERT KEYLVQQGRE
EDYIEMKADE DAAYDQELDV DLSKLVPMTA KPHSPDAVVP VKELEGMKVN QVVIGSCTNS
SFADMMKAAK ILKGRKVANH VSLVIAPGSS SILAMLSQNG ALADMVQAGA RILECGCGPC
IGMGQAPLSK GVSLRTINRN FKGRSGTNDA SVYLVSPEIA ALSAIKGYMS EEFKDDMYLD
EVPNTPFVKN RNFFIDEYDE NNEVYMGPNI KPVPRGDKIS DEISGKVVLK VGDNISTDHI
VPSDSKLLPY RSNVPHLAKF SFSKVDPEFY DRAIANDGGF IVGGDNYGQG SSREHAALVP
NYLKIKAIFA VSFARIHRSN LINNGILPLV IEAKDQDFFN DQDNYKIVNI KEVVDHNGKV
KVINETTNES IEASLTLSPR EKVMINYGGL LNAIKELGGE F
//
