ID A0A1I0IZV8_9BACL Unreviewed; 846 AA.
AC A0A1I0IZV8;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=SAMN03159358_3171 {ECO:0000313|EMBL:SEU02908.1};
OS Paenibacillus sp. NFR01.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1566279 {ECO:0000313|EMBL:SEU02908.1, ECO:0000313|Proteomes:UP000198735};
RN [1] {ECO:0000313|EMBL:SEU02908.1, ECO:0000313|Proteomes:UP000198735}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NFR01 {ECO:0000313|EMBL:SEU02908.1,
RC ECO:0000313|Proteomes:UP000198735};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
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DR EMBL; FOIK01000002; SEU02908.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I0IZV8; -.
DR STRING; 1566279.SAMN03159358_3171; -.
DR OrthoDB; 9801077at2; -.
DR Proteomes; UP000198735; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 3.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 3.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000198735};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 199..301
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 671..759
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 762..843
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 846 AA; 95477 MW; 6C6A62C990CD97D7 CRC64;
MKQKHLVLAD WQFKACLEHE WLPAKVPGTV HTDLLANGKI TEPFYGTNEH DLQWIDKLDW
EYRTTLELGE EWLQLPVAEL QFDGLDTYAD VFVNGVHALA ADNMFRAWTV DVKGLIKAGP
NEILVKFRSP VNEDLPKLEQ LGYALPAPND QSELGGLEGQ QISVFARKAP YHYGWDWGPR
FLTAGIWREA VLKGREAAEI SDLYIRQNEV KAQAARLTAV AEIDAPEAWE GTLRITADGQ
EWTRAVSLQP GKQTVELDLV IDQPRLWWCR GLGEPELYEF SAALLAQETV VTEANVTTGL
REIKLIRKPD ARGTSFYFEV NGVPVFAKGA NHIPNDSFIT EVTEERYRHE IESAALSGMN
MLRVWGGGFY EEEVFYRLCD EYGLLVWQDF MFACSMYPGD AEFLESVRLE AEYNVRRLRN
HPSIALWCGN NEIDSAWSHY EEDAGWGWKR NFSSELREKL WSDYQEIFHV ILPKAVAELH
ADIAYWPSSP MRGVTGDINQ HATGITGEGD IHYWGVWHAI EPFENYNVKV GRFMSEYGFQ
SFPELKSVLS YAEDADMELE SKVMLAHQKN GRGNLLIKEY MDIYLPKPKN FPSFLYMSHI
LQADAIRMAI ESHRRNKPYC MGTLYWQMND CWPVASWAGM DYYGRWKALQ YTVKESFRDL
LLSIDGTDGE NIHVHAVSDL RKPVEAELKL GLYSFGGELL KAIAQPVMLA ADSAAVVFSI
SAAELLGSTA VDQAVLVATL EQGGEELARK LHYFAAPKEI RLATPELTIQ EVPGSGGLKY
TVSSNVLARG VYLTAEDEGI FSDNFFDLLP GQPKTVSFLL RSGGEQHFVP AAPKGLAVSS
MADYIE
//
