ID A0A1I0L090_9BACL Unreviewed; 1175 AA.
AC A0A1I0L090;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=SAMN03159358_0035 {ECO:0000313|EMBL:SEU31463.1};
OS Paenibacillus sp. NFR01.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1566279 {ECO:0000313|EMBL:SEU31463.1, ECO:0000313|Proteomes:UP000198735};
RN [1] {ECO:0000313|EMBL:SEU31463.1, ECO:0000313|Proteomes:UP000198735}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NFR01 {ECO:0000313|EMBL:SEU31463.1,
RC ECO:0000313|Proteomes:UP000198735};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; FOIK01000010; SEU31463.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I0L090; -.
DR STRING; 1566279.SAMN03159358_0035; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000198735; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:SEU31463.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000198735}.
FT DOMAIN 628..789
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 806..964
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1175 AA; 133093 MW; CE89BAE0D9334A41 CRC64;
MLQRLIEAFS KDSDFQSISR GLAAGMKEQL ISGLSGSARQ IMLAALYEDI KRPLLVVTHN
MFSAQKIAED LQEALSSDQV LLYPANELVA AEAAVSSPET LAQRIDVLTK CAQGFRGIVV
VPYSGVRRLL PSAPVMGKAQ LTVTQEGTLS LDEFLHAMIE MGYERVERVE NRGEISVRGG
IIDFYPMTSQ LAYRVELFDD EVDSIRTFDP ADQRSIDKVR SVTITPAKEI IADRERLDAA
AVQAAAMMER QLEKMTDRQA KLRLREEIGR ELEMLREGTY FPEIYKYISL LYPERTHLYD
YMAKDTVLVL DEPARLLETA KQLERDESEW NLHLLQNGKT LPDLHLSVDN DTIMYRRPFQ
SLFMSIFLRQ VPHIQPQNIL GFISRGMQDF HGQMNVLKTE MERWHKSGVQ VVMLASGEER
IERVRRVLYD YGIEEPTILQ GNLGSGFEMP SIHLAVITEG EMFSQKQRKA RKASSRGMDN
AERIKSYTEL KIGDYVVHQN HGIGKYMGIG TLEVSGIHRD YMHIMYAGGD KLSVPIDQID
LIQKYVGSED KEPKVYKLGG NEWTRVTSKV RSSVQDIADD LIKLYAERQS ALGYGFEKDT
QEQREFEEMF PYEETRDQLR AIEEIKKDME QNRPMDRLLC GDVGYGKTEV AIRAAFKAAI
EGKQVAVLVP TTILAQQHYE TFRERFANYP LNIQVLSRFR SRKEQNETLK GVRQGTVDVI
IGTHRLLSQD MVFKDLGLLI VDEEQRFGVT HKEKLKKLKT NVDVLTLTAT PIPRTLHMSM
LGVRDLSVIE TPPENRFPVQ TYVVEHSQTL IREAVERELA RGGQVYYLYN RVQGIQEMAA
QISMLVPEAR VGIGHGQMSE GELEKTILDF LDGEYDVLVS TSIIETGVDI PNVNTLIVHD
ADKMGLSQLY QLRGRVGRSN RIAYAYFTYQ RDKVLTEVAE KRLQSIKEFT ELGSGFKIAM
RDLSIRGAGN LLGAEQHGFI ASVGFDLYSQ MLAEEIRKRK VTVLGEEDTS LKQGNTVIDL
SIDAYLPSEY IYDSIQKIEI YKKVAAVSSF DDAAELEDEL LDRFGELPES VVNLLSVARL
KVYGKLYGLE SIIRRGDEAV LNFHEGAAAA FDTAKLAKVG NSFERRVQFD RDAKAAIRLK
TKNLNDKELL ELLESFLAAA KQSLKSKGEL HNAVK
//
