ID A0A1I0MKE5_9FLAO Unreviewed; 490 AA.
AC A0A1I0MKE5;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:SEV88330.1};
GN ORFNames=SAMN05421841_0009 {ECO:0000313|EMBL:SEV88330.1};
OS Chryseobacterium wanjuense.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Chryseobacterium.
OX NCBI_TaxID=356305 {ECO:0000313|EMBL:SEV88330.1, ECO:0000313|Proteomes:UP000199469};
RN [1] {ECO:0000313|EMBL:SEV88330.1, ECO:0000313|Proteomes:UP000199469}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17724 {ECO:0000313|EMBL:SEV88330.1,
RC ECO:0000313|Proteomes:UP000199469};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR EMBL; FOIU01000001; SEV88330.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I0MKE5; -.
DR STRING; 356305.SAMN05421841_0009; -.
DR OrthoDB; 9806009at2; -.
DR Proteomes; UP000199469; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT DOMAIN 3..422
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 232
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 262
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 195
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 201
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 236
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 302
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 490 AA; 56601 MW; FBE8DBABF22DF5D6 CRC64;
MNETMIQFFH WYSEGDGKLW KEAEKHAQYL ADLGITSVWL PPAYKGATGG HSTGYDAYDL
YDLGEFDQKG TIPTKYGTKE DYIQAIKALK EKNIQVIVDI VLGHKGGGDE LESFKAVKVD
EENREKIISD EIEIQSYTKF TFPGRGKKYS DFEWNFTCFS GVDYAEGMDS HIFKIKNEYG
TDWEEMIDDE KGNYDYLMYN DIEHRNPFVR EELNNWAKWY FDETDFDGVR LDALKHISFD
FYKEWLTLLR SNSGKNIFAV GEYWAPGYLN LLQKYIEVTE GCMSLFDSSL QNNFHTASKE
GGSYDLRRIF DGSLTQADPL HSVSLVDNHD TQPLQDLEAP VELWFKPLAY AMILLRKDGY
PCVFYPDLYG AHYVDKDREG NDQEIFLDKV DKIEELLKAR KDHAYGEQRD YFEDANCLGW
VREGDEEHSG CAVVLSNKDA YEKPMEMGEK YIGKTFYDLL GRFENKVTID ENGWGSFPVP
AGNVSVWVAE
//
