ID A0A1I0QZ94_9BACT Unreviewed; 667 AA.
AC A0A1I0QZ94;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=SAMN05216290_2937 {ECO:0000313|EMBL:SEW32979.1};
OS Roseivirga pacifica.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Roseivirgaceae;
OC Roseivirga.
OX NCBI_TaxID=1267423 {ECO:0000313|EMBL:SEW32979.1, ECO:0000313|Proteomes:UP000199437};
RN [1] {ECO:0000313|Proteomes:UP000199437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.12402 {ECO:0000313|Proteomes:UP000199437};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; FOIR01000002; SEW32979.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I0QZ94; -.
DR SMR; A0A1I0QZ94; -.
DR STRING; 1267423.SAMN05216290_2937; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000199437; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000199437};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..667
FT /note="pullulanase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011623478"
FT DOMAIN 131..567
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 667 AA; 75181 MW; 029FA03A88CB9BC1 CRC64;
MKMKAFKTIL GLTLLFMAMA CNTTPKYETL EDYPIYDGND LGMTYTPAST QLKIWSPAAQ
SVKVKLYEKG DGDNLIDEFK MKRDKQGVWS YKLKGDHAGE YYTYQVQQNG QWLQEKPDLY
AVAVGVNGQR SMIIDLKNTN PEGWEEDQKP ALANYNDIII YELQIRDVSI SPNSGITQKG
KYAGLVETGT KSPEGLSTGL DHIKDLGVTH VHLLPTFDHR SIDETKLDEP QYNWGYDPLN
YNVPEGSFST NPYDGAVRIK EFKQMVKTLH ENGIRVILDV VYNHTGQTED SNFNQLVPDY
YYRQNAEGGF SDASACGNET ASERAMMRKY IIESVAYWAK EYHLDGFRFD LMGIHDIETM
NEVSRTLREI DPSIFVYGEG WTAGSSPLPQ EEQALKANTY KLEQVAAFSD DLRDGLKGSV
FEEKEGGFVS QTTGLKESIK FGIVASTQHP QVNYDSVNYS NAPWAAEPYQ TISYVSCHDN
NTLWDKLLLS RPDASEADRI KMHKLAETIV LTSQGVPFIH AGMEMLRSKD GVENSYESPD
AINQIDWSRK AQYKEVYEYY KGIIALRKAH PAFRMTSTKD IQSNLEFLDY AGDNFIGYRL
KNNANGDQWE EILVLLNGAD TNKTISLPAG NWTLVADGSK VSEKGLRKLS SKTLTVPGIT
AYILKKD
//
