ID   A0A1I1GIZ9_9LACT        Unreviewed;       444 AA.
AC   A0A1I1GIZ9;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 22.
DE   SubName: Full=Glucosylceramidase {ECO:0000313|EMBL:SFC11524.1};
GN   ORFNames=SAMN04488102_103103 {ECO:0000313|EMBL:SFC11524.1};
OS   Alkalibacterium subtropicum.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
OC   Alkalibacterium.
OX   NCBI_TaxID=753702 {ECO:0000313|EMBL:SFC11524.1, ECO:0000313|Proteomes:UP000199612};
RN   [1] {ECO:0000313|EMBL:SFC11524.1, ECO:0000313|Proteomes:UP000199612}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 23664 {ECO:0000313|EMBL:SFC11524.1,
RC   ECO:0000313|Proteomes:UP000199612};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC       {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
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DR   EMBL; FOLT01000003; SFC11524.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I1GIZ9; -.
DR   STRING; 753702.SAMN04488102_103103; -.
DR   OrthoDB; 9806701at2; -.
DR   Proteomes; UP000199612; Unassembled WGS sequence.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:InterPro.
DR   GO; GO:0006665; P:sphingolipid metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR033452; GH30_C.
DR   InterPro; IPR001139; Glyco_hydro_30.
DR   InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR   PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF02055; Glyco_hydro_30; 1.
DR   Pfam; PF17189; Glyco_hydro_30C; 1.
DR   PRINTS; PR00843; GLHYDRLASE30.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361188};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          48..376
FT                   /note="Glycosyl hydrolase family 30 TIM-barrel"
FT                   /evidence="ECO:0000259|Pfam:PF02055"
FT   DOMAIN          379..439
FT                   /note="Glycosyl hydrolase family 30 beta sandwich"
FT                   /evidence="ECO:0000259|Pfam:PF17189"
SQ   SEQUENCE   444 AA;  50058 MW;  B03143990FE610CC CRC64;
     MTKRTVTAVQ SVKADNSFWQ PVSIKEVTDN STTHVTVDST QTYQTWMGFG GALTESATYT
     MDQLPAEKKE SILQAYYNKE NGLGYTLGRL HIASCDFSLE NYDYIEENDE TLETFNMDRE
     EKWVLPTLRR AQEIAGEPLT LVASPWSPSA WMKTNGERNN GGKLKKEYYT LWAQFIARYL
     KEMKDKGFET FAITVQNEPA ATQTWDSCEF TAEEEAEMVK VLSGVFKEEN LDVNIIIWDH
     NRDIIFERAD TVLSDPEANK GVWGVGNHWY MSEDFEALSK VHDKYPDKHM IFTEGCIEGG
     VQLGAWHTGE RYARNIIGDM NNWLEGFLDW NIVLDEQGGP NHVGNYCDAP IIADTKEKVA
     HYNSSYYFIG HLSKYIRPGA ERVDVTLSGD DLSVVAFKNE DGSLVTVLLN ETDKASDLVL
     SLDNEGAAVH VPAHSITTVV SEQA
//