ID A0A1I1JL78_9LACT Unreviewed; 398 AA.
AC A0A1I1JL78;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=trans-2-enoyl-CoA reductase (NAD(+)) {ECO:0000256|ARBA:ARBA00011983};
DE EC=1.3.1.44 {ECO:0000256|ARBA:ARBA00011983};
GN ORFNames=SAMN04488102_10849 {ECO:0000313|EMBL:SFC49317.1};
OS Alkalibacterium subtropicum.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
OC Alkalibacterium.
OX NCBI_TaxID=753702 {ECO:0000313|EMBL:SFC49317.1, ECO:0000313|Proteomes:UP000199612};
RN [1] {ECO:0000313|EMBL:SFC49317.1, ECO:0000313|Proteomes:UP000199612}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 23664 {ECO:0000313|EMBL:SFC49317.1,
RC ECO:0000313|Proteomes:UP000199612};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2,3-saturated acyl-CoA + NAD(+) = a (2E)-enoyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:18177, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58856, ChEBI:CHEBI:65111; EC=1.3.1.44;
CC Evidence={ECO:0000256|ARBA:ARBA00001615};
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
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DR EMBL; FOLT01000008; SFC49317.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I1JL78; -.
DR STRING; 753702.SAMN04488102_10849; -.
DR Proteomes; UP000199612; Unassembled WGS sequence.
DR GO; GO:0050343; F:trans-2-enoyl-CoA reductase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR024906; Eno_Rdtase_FAD-bd_dom.
DR InterPro; IPR024910; Enoyl-CoA_Rdtase_cat_dom.
DR InterPro; IPR010758; Trans-2-enoyl-CoA_reductase.
DR PANTHER; PTHR37480; ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADH]; 1.
DR PANTHER; PTHR37480:SF1; ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADH]; 1.
DR Pfam; PF07055; Eno-Rase_FAD_bd; 1.
DR Pfam; PF12242; Eno-Rase_NADH_b; 1.
DR Pfam; PF12241; Enoyl_reductase; 1.
PE 4: Predicted;
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 84..319
FT /note="Trans-2-enoyl-CoA reductase catalytic"
FT /evidence="ECO:0000259|Pfam:PF12241"
FT DOMAIN 326..383
FT /note="Enoyl reductase FAD binding"
FT /evidence="ECO:0000259|Pfam:PF07055"
SQ SEQUENCE 398 AA; 44455 MW; 504A73BB7BDD7296 CRC64;
MVEFEQKLRG NIAVSVNPLG RKQQVLNHID YVKSQGSYEG PKKVLIIGAS SSYGLATRIS
LAFGAGADTI GVSFEKGPKS ERNLGTAGWY NNIFFKEAAE KEGLIAKNFV QDAFSNETKQ
EVINYIKNEF GGKVDLVVYS LASGRRTDSE TGETYTSSIK AIGEPVVGPN INMQKEEYYT
ETLEPATEQE IEDTVKVMGG EDWERWMKAL KEADVLADGV LTTNYSYLGS ELNHPYYGHG
TLGRAKADCD EKAENINELL KDIDGKAQIV VATAVTTKAS SVIPFFPVYC IGLYKVMSEK
GTHETPIMHM DRIYREMIYG NKSEYDEQGR LRPDSWELDS DTQAQTKALI GEINEENFTT
DKTAYALFYK EFSNLSGFMV DNCEEQNVTI EELKALEY
//
