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Entry: A0A1I1VU89_9PROT
LinkDB: A0A1I1VU89_9PROT
Original site: A0A1I1VU89_9PROT 
ID   A0A1I1VU89_9PROT        Unreviewed;       243 AA.
AC   A0A1I1VU89;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   RecName: Full=Dual-specificity RNA pseudouridine synthase RluF {ECO:0000256|ARBA:ARBA00039989};
DE            EC=5.4.99.21 {ECO:0000256|ARBA:ARBA00038922};
DE   AltName: Full=23S rRNA pseudouridine(2604) synthase {ECO:0000256|ARBA:ARBA00041697};
DE   AltName: Full=Ribosomal large subunit pseudouridine synthase F {ECO:0000256|ARBA:ARBA00042890};
DE   AltName: Full=rRNA pseudouridylate synthase F {ECO:0000256|ARBA:ARBA00042843};
DE   AltName: Full=rRNA-uridine isomerase F {ECO:0000256|ARBA:ARBA00043147};
DE   AltName: Full=tRNA(Tyr) pseudouridine(35) synthase {ECO:0000256|ARBA:ARBA00041420};
GN   ORFNames=SAMN05428977_100167 {ECO:0000313|EMBL:SFD85618.1};
OS   Nitrosomonas sp. Nm166.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=1881054 {ECO:0000313|EMBL:SFD85618.1, ECO:0000313|Proteomes:UP000199102};
RN   [1] {ECO:0000313|EMBL:SFD85618.1, ECO:0000313|Proteomes:UP000199102}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nm166 {ECO:0000313|EMBL:SFD85618.1,
RC   ECO:0000313|Proteomes:UP000199102};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA;
CC         Xref=Rhea:RHEA:38875, Rhea:RHEA-COMP:10093, Rhea:RHEA-COMP:10094,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.21;
CC         Evidence={ECO:0000256|ARBA:ARBA00036535};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr);
CC         Xref=Rhea:RHEA:60556, Rhea:RHEA-COMP:15607, Rhea:RHEA-COMP:15608,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000256|ARBA:ARBA00036390};
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DR   EMBL; FONE01000001; SFD85618.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I1VU89; -.
DR   STRING; 1881054.SAMN05428977_100167; -.
DR   OrthoDB; 9807213at2; -.
DR   Proteomes; UP000199102; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02555; PSSA_1; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR   PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR   PANTHER; PTHR47683:SF2; S4 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS50889; S4; 1.
PE   4: Predicted;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199102};
KW   RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT   DOMAIN          4..71
FT                   /note="RNA-binding S4"
FT                   /evidence="ECO:0000259|SMART:SM00363"
SQ   SEQUENCE   243 AA;  27462 MW;  5D5397D96294EF5C CRC64;
     MEKIRLSKLM SEQGICSRRE ADRYIELGWV FVDGVQISEL GTKIYPTQKI QLNKAAQVQQ
     TSLVTILLNK PIGYVSGQPE PGYQPAVMLI KPENQFVPKQ PAKRFQPTHL KNLAPAGRLD
     IDSQGLLVLT QDGRIAKQLI GKNSDIEKEY LVRVEGPLPA AKLALLNHGL SLDGQALKPA
     QVSWQNQDQL RFILFEGKKR QIRRMCELVN LKVTGLKRVR IGKVKLGNLP EGQWRYLDDS
     EKF
//
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