ID A0A1I2E720_9ACTN Unreviewed; 708 AA.
AC A0A1I2E720;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=3-hydroxyacyl-CoA dehydrogenase {ECO:0000313|EMBL:SFE88503.1};
GN ORFNames=SAMN05216251_10654 {ECO:0000313|EMBL:SFE88503.1};
OS Actinacidiphila alni.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Actinacidiphila.
OX NCBI_TaxID=380248 {ECO:0000313|EMBL:SFE88503.1, ECO:0000313|Proteomes:UP000199323};
RN [1] {ECO:0000313|EMBL:SFE88503.1, ECO:0000313|Proteomes:UP000199323}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 4.3510 {ECO:0000313|EMBL:SFE88503.1,
RC ECO:0000313|Proteomes:UP000199323};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
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DR EMBL; FONG01000006; SFE88503.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I2E720; -.
DR STRING; 380248.SAMN05216251_10654; -.
DR OrthoDB; 9771883at2; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000199323; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000199323}.
FT DOMAIN 339..519
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 523..605
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 708 AA; 75195 MW; D12BDA6FEE5DABAC CRC64;
MSSTTDLLKG AAERFPDEVV TQAQVRHIEL PGAGRFALIT LDNGFDHTKP TTFGPQSLAN
LDAAIDQVEK EAAEGTITGV GITGKPFIYA VGADLKGVEV LRTRDEALTI GKSGHDVFKR
LQTLAVPTFA YYNGAAMGGG VEVGLHCTYR TVSASVPAFS LPEVFLGLVP GWGGCVLLPN
LIGADRAVGV IIENSLNQNR QLKGAQVYEL GIADALFEAA DFLEQSLAWT AAVLKGELEV
VRPEVDRGEG WDQAVARGRA IADSKVHGAA PAAYRALDIV AAAKNGDLRH GFDAEDAALA
DLIMGGELRS GIYAFNLVQK RGKRPVGAPD KALARPVSKV GVVGAGLMAS QLALLFARRL
EVPVVLTDID QERIDKGVGY VHGEIDKLLA KGRVGQDKAN RLKSLVSGHL DKATAFGDAD
FVIEAVFEEL GVKQQVFAEL EAVVRPDAIL ATNTSSLSVS EMAAKLEHPE RVVGFHFFNP
VAVLPLLEIV RGERTDDPSL ATAFAVAKKL KKTAVLVKDA PAFVVNRILT RFMGEIQNVI
DEGTPVAVAE QAVEPLGLPM SPLVLLELVG PAIGLHVSET LHRAFPDRFT VSENLAAVVK
AGKRGFYVYD SGKPELDPEV AALLKQGDTV LTEAQVRDRV LDAVAQEIGL MLDEGVVAEA
QDIDLCLITG AGWPFHLGGI TPYLDREGVS QRVNGKPFLA PGVASVPA
//
