ID A0A1I2GNF6_9BACT Unreviewed; 1254 AA.
AC A0A1I2GNF6;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SAMN04488541_101985 {ECO:0000313|EMBL:SFF19494.1};
OS Thermoflexibacter ruber.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Thermoflexibacteraceae;
OC Thermoflexibacter.
OX NCBI_TaxID=1003 {ECO:0000313|EMBL:SFF19494.1, ECO:0000313|Proteomes:UP000199513};
RN [1] {ECO:0000313|EMBL:SFF19494.1, ECO:0000313|Proteomes:UP000199513}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GEY, DSM 9560 {ECO:0000313|Proteomes:UP000199513};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
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DR EMBL; FONY01000019; SFF19494.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I2GNF6; -.
DR STRING; 1003.SAMN04488541_101985; -.
DR OrthoDB; 32195at2; -.
DR Proteomes; UP000199513; Unassembled WGS sequence.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR011639; MethylTrfase_TaqI-like_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF07669; Eco57I; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:SFF19494.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000199513};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 864..971
FT /note="Type II methyltransferase M.TaqI-like"
FT /evidence="ECO:0000259|Pfam:PF07669"
FT DOMAIN 1078..1195
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
FT COILED 807..838
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1254 AA; 145423 MW; 20B9EC9F238ADCF7 CRC64;
MILKELTPRK ALNKAFLKLK PSRTEIDNFK TNLITLLERV NDTESEEFHK NLLIDFLKKT
YYDPHHFLNT KGRNDLVIHN GSTAKSPVGV IIEAKKPSNK VEMLTRDNIN KKAFQELILY
YLRERVTHKN LEIKHLAATN IYEWFIFDAM LLDRLFAQNK NLVKLFNDFE AGRLADTKTD
FFYKQVAEPF IGEISAEIEF TYFSFPRVNS GAIDDNDLIP LFKILSPEHL LKLPFANDSN
SLDKRFYSEL LHIIGLTEIK EGNKKLIERN KAGERHTGTI LENAVIQLDS LDKMSRLEKP
SQFGKTEQER LFNVALELSI TWVNRILFLK LLEAQLITYH KGDASYSFLH SDKIKSYDDL
NSLFFQVLAR QHDERNEEVK KIFEKVPYLN SSLFEPTDTE QQTIFISNLK DNINIPIFSQ
TVLKDPQGKK RTGKLNTLQY FLEFLDAYNF GAEGSEEIQE DNKTLINASV LGLIFEKING
YKDGSFFTPG FITMYMCRET IRRAVVQKFK EAGYQLSESL ELSRSLTEIY NQIKDIKEAN
KIVNTLKICD PAAGSGHFLV SALNEIIAIK NDLGILCDFE GRPLKNYWVE VVNDELFITE
KETGEEFCYI APMPRTEKAI KHTFGGDTSN YKASQLPERQ VVQQSLFKEK QAIIENCLFG
VDINPNSVKI CRLRLWIELL KNAYYSPESN FTELETFPNI DINIKCGNSL VSRFAIDADL
SKSLRSLKWD IKTYRQLVQS YLHATDKEAK RGFEKLINDI KKDFSIQISN SDPKKTRLSK
LAYELYNRFT GMMLFEPEVP YGDNKSDKNL EAKRKEEQGK IEEEMEKLRR EIDEIENNKI
FENAFEWRFE FPEVLNDDGD FVGFDVVIGN PPYGVSIKEK TEREYLVSNL SKVPDYEIYY
WFINKGHHIL KQNGIISYII PNTILFNVFA QSYRLSLFDN WTINEILDCT NFNIFEDATV
RNIIFQFIKS DSKKNTLGYK NTANIENFEN LISCKTLTIS KEIAVSNIQN WGLVFKLDRE
TLNVVEKIRS EKKPLIELFP ETSQGLIAYD KYQGQNAEII KNRAYHHFSN PNNKFKRWLY
GGDITRYSVK WNGKEFIDYC DGIANPREPK YFKGKRLLIR EITNPRIFSA ITTEELYNDP
AIIIVKENPK SYPIECLLGI FNSKLATFYH FNSSPKATKG AFPKILVYDV NNFPLPKTID
KVTQEKIENL VNQILTKKAE DNSNDTADLE NQIDQLVYEL YGLTEAEIKM IEQS
//
