ID A0A1I2J5S2_9ACTN Unreviewed; 1042 AA.
AC A0A1I2J5S2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=phospholipase C {ECO:0000256|ARBA:ARBA00012018};
DE EC=3.1.4.3 {ECO:0000256|ARBA:ARBA00012018};
GN ORFNames=SAMN05216251_116124 {ECO:0000313|EMBL:SFF50045.1};
OS Actinacidiphila alni.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Actinacidiphila.
OX NCBI_TaxID=380248 {ECO:0000313|EMBL:SFF50045.1, ECO:0000313|Proteomes:UP000199323};
RN [1] {ECO:0000313|EMBL:SFF50045.1, ECO:0000313|Proteomes:UP000199323}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 4.3510 {ECO:0000313|EMBL:SFF50045.1,
RC ECO:0000313|Proteomes:UP000199323};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycerol + H(+) + phosphocholine; Xref=Rhea:RHEA:10604,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023550};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10605;
CC Evidence={ECO:0000256|ARBA:ARBA00023550};
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000256|ARBA:ARBA00004191}.
CC -!- SIMILARITY: Belongs to the bacterial phospholipase C family.
CC {ECO:0000256|ARBA:ARBA00009717}.
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DR EMBL; FONG01000016; SFF50045.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I2J5S2; -.
DR Proteomes; UP000199323; Unassembled WGS sequence.
DR GO; GO:0034480; F:phosphatidylcholine phospholipase C activity; IEA:UniProtKB-EC.
DR GO; GO:0016042; P:lipid catabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.200; -; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 2.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR017767; Bact_PC-PLC.
DR InterPro; IPR007312; Phosphoesterase.
DR InterPro; IPR008475; PLipase_C_C.
DR InterPro; IPR006311; TAT_signal.
DR NCBIfam; TIGR03396; PC_PLC; 1.
DR PANTHER; PTHR31956:SF8; ACID PHOSPHATASE PHOA (AFU_ORTHOLOGUE AFUA_1G03570); 1.
DR PANTHER; PTHR31956; NON-SPECIFIC PHOSPHOLIPASE C4-RELATED; 1.
DR Pfam; PF04185; Phosphoesterase; 1.
DR Pfam; PF05506; PLipase_C_C; 2.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Reference proteome {ECO:0000313|Proteomes:UP000199323};
KW Secreted {ECO:0000256|ARBA:ARBA00022512}; Signal {ECO:0000256|SAM:SignalP};
KW Virulence {ECO:0000256|ARBA:ARBA00023026}.
FT SIGNAL 1..32
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 33..1042
FT /note="phospholipase C"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011595011"
FT DOMAIN 529..609
FT /note="Bacterial phospholipase C C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05506"
FT DOMAIN 616..694
FT /note="Bacterial phospholipase C C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05506"
FT REGION 179..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 487..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1042 AA; 111276 MW; A316CD82F677192E CRC64;
MSPELSRRTL LGSAAGAALL SALPMSVRTA LAAPARPGSL EDIAHVVVLM QENRSFDHYY
GTMQGVRGYG DRTAVRGVNG RPVWAQPDPS RAEGHLLPFA MNAAHTNAYQ QGAPAFGFTD
SMNARNDGLA DGYVTRRGGG WLGQGYYEPA DMPFYNALAS VFTICDNYHA AMETSTNPNR
EHFMTGTSGG TVRDTPVTDN TETSAGYEWT TYAERLEAAG ISWKTYQAQE NFDDNALAWF
APFHRAKPGE PLYERGMRRA GDPAQAGDPW AMGDALVAAF AADVAADALP QVSWLVAPAA
LSEHASYAPP DGEDLTARLL AALADHPAVF AKTAFILNYD EHGGFFDHVL PPVPPMGAGR
GKSTVSTDGE IVVRVTKSGS TYHRVVGYDG RYRVKAADGS LTWSDTLPAG ESVVAGPFPL
GLGIRVPMIV ASPWTRGGVV DSTVYDHTSV IKFLEKRFGV REPNISEWRR AVTGDLTSVF
DFSGDEPKWP SLPDTSGNRR KVTDSGKLPA PTVPSPQVPP VQQRGSRAAR PSPYALRMRS
HVRGASLELD FVNLGTQAAV FAAYPAPGSR PRFFTVGARD RLSDAWQIDA DGYDLRVNGP
NGSLWHFRGG AARLEAQLTE DPVARHLEIE LRNDERGPRT FRIGDLAYRG GVHEMRVGGG
ATRSITLGVD HDGWYDVAVT DADDPFFLRR FAGKVADHRP GVTDPAMGAP DALTAEVRLS
AASPAIDEPV VLPGRPSTVT AVLTATVAVT KLEAAPVVPA GWEVRTVTAP PARLSAGASA
TAVWQVTPPA ALPSDTTHRV LVTARGTAGN RLALADCEVA ARTAPSMDGY LLGEDFESLA
GQLKPAVTLP VPADVLGWTA TAPACWSVVN AAGMPQGSVE EQGWTFMTKR FWTASDSQDR
GNFSRALGII AVADPDDWDD HGSPSGKGTF DSTLVSPAVP VPAGATVLHL GFDSHYRQEA
PQTATVSAVF DTGEETVLLT YSGAATGNDN AGHDAENQLV TRTVDVPSGA RTVTLKFRMF
NAGNNWYWAI DHIRLGTRPV TD
//
