ID A0A1I2MVH5_9CLOT Unreviewed; 555 AA.
AC A0A1I2MVH5;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=B12-binding domain-containing radical SAM protein {ECO:0000313|EMBL:PWL54851.1};
DE SubName: Full=Radical SAM superfamily enzyme YgiQ, UPF0313 family {ECO:0000313|EMBL:SFF95482.1};
GN ORFNames=DBY38_03445 {ECO:0000313|EMBL:PWL54851.1}, SAMN04487885_11735
GN {ECO:0000313|EMBL:SFF95482.1};
OS Clostridium cadaveris.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1529 {ECO:0000313|EMBL:SFF95482.1, ECO:0000313|Proteomes:UP000182135};
RN [1] {ECO:0000313|EMBL:SFF95482.1, ECO:0000313|Proteomes:UP000182135}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NLAE-zl-G419 {ECO:0000313|EMBL:SFF95482.1,
RC ECO:0000313|Proteomes:UP000182135};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PWL54851.1, ECO:0000313|Proteomes:UP000246114}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CIM:MAG 903 {ECO:0000313|EMBL:PWL54851.1};
RA Jeraldo P., Boardman L., White B.A., Nelson H., Goldenfeld N., Chia N.;
RT "The uncultured portion of the human microbiome is neutrally assembled.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; QAMZ01000018; PWL54851.1; -; Genomic_DNA.
DR EMBL; FOOE01000017; SFF95482.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I2MVH5; -.
DR STRING; 1529.SAMN04487885_11735; -.
DR eggNOG; COG1032; Bacteria.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000182135; Unassembled WGS sequence.
DR Proteomes; UP000246114; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000182135}.
FT DOMAIN 1..133
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 170..393
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 555 AA; 65400 MW; FD05FDEED58883CC CRC64;
MKTLLVGINS KFIHSNLAVR YLKAYTKKLN YESEILEVTI NDSIENIVRA IILKKPNIVG
FSTYIWNLEY VEKISRIIKE IDSNIEIFYG GPEVSYDSLS FLSNSAGDYV IEGEGEETFY
EFMKNKINGD SLNSIKGLYI KNQDGSINYA GQRALIDMNS IVFPYNDKDD LKNKIVYYEA
SRGCPFRCKY CLSSTTHGVR FLPVERVKRE LKFLIDQDVK LIKFVDRTFN CNSKFAMEIW
RFLIEEGSNT CFHFEISADI LKHEEIELLA KAKNGQFQFE VGVQTTNNEV LKNINRFVNF
DDIKEKVIEL ESIKNIKQHL DLIAGLPGED LNSFKKSFDD VYSIKPEEIQ LGFLKLLKGS
SMREEAEKWG MKFSPYPPYE ILRTNDISYE EILILKNTEK VVDKYYNSRK FTNIINFLIK
KWDSPFRFFY ELGQYFYDKG YFNINISNNE YYKVFLDFNM EMLREDEEPL KSIVRYDYLS
FNKKRGIPDF LKSDITKTEE RVIKENIKND IGTWDNSWTI DKFVIDMEEF EKNKKVIIKN
IYTIFKGIDR IFVEY
//
