ID A0A1I2SV67_9FIRM Unreviewed; 555 AA.
AC A0A1I2SV67;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=SAMN05216356_11342 {ECO:0000313|EMBL:SFG56488.1};
OS Oribacterium sp. WCC10.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Oribacterium.
OX NCBI_TaxID=1855343 {ECO:0000313|EMBL:SFG56488.1, ECO:0000313|Proteomes:UP000199160};
RN [1] {ECO:0000313|EMBL:SFG56488.1, ECO:0000313|Proteomes:UP000199160}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WCC10 {ECO:0000313|EMBL:SFG56488.1,
RC ECO:0000313|Proteomes:UP000199160};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; FOPH01000013; SFG56488.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I2SV67; -.
DR STRING; 1855343.SAMN05216356_11342; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000199160; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:SFG56488.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}.
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 555 AA; 59705 MW; 655E26089C7F1893 CRC64;
MKSDIEIAQQ ATMERITDVA ARYGIDNDDL EQYGKYKAKL SDELWTKIKN RPNGKLVLVT
AINPTPAGEG KTTTSIGLSD ALNYVGKKTV VALREPSLGP CFGIKGGAAG GGYAQVVPME
DLNLHFTGDF HAITSANNLL AALLDNHIQQ GNELRIDLKN INWKRCEDMN DRQLRNIVIG
MGKKTDGVVR EDHFVITVAS EIMAVLCLAD DMDDLKKRLA RIIVAYDVDG KPVTADDLKA
TGSMAALLKD AIKPNIIQTL EHNMAIVHGG PFANIAHGCN SVQATRFALK LGDIAVTEAG
FGADLGAEKF FDIKCQMAGF KPDAVVLVAT VRALKYNGGV PKAELGSENL DALKKGIVNL
EKHIENLQTY GVPVVVAINQ FASDTDAELG FVENFCREKG ADFALSKVHG EGGKGGVELA
NTVIRTLETK ESHFTPVQNL SDSLYDKINA VATKIYGADG VDFDSKAKKS IDRLTELGFG
NVPVCMAKTQ YSLSDDPKKL GRPSGFRITV RDVYVSAGAG FIVCLTGDIM TMPGLPKKPA
AFDIDVVDGK IVGLF
//
