ID   A0A1I2VX73_9SPHI        Unreviewed;       810 AA.
AC   A0A1I2VX73;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Penicillin amidase {ECO:0000313|EMBL:SFG92376.1};
GN   ORFNames=SAMN04489864_103248 {ECO:0000313|EMBL:SFG92376.1};
OS   Pedobacter insulae.
OC   Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC   Sphingobacteriaceae; Pedobacter.
OX   NCBI_TaxID=414048 {ECO:0000313|EMBL:SFG92376.1, ECO:0000313|Proteomes:UP000199666};
RN   [1] {ECO:0000313|EMBL:SFG92376.1, ECO:0000313|Proteomes:UP000199666}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18684 {ECO:0000313|EMBL:SFG92376.1,
RC   ECO:0000313|Proteomes:UP000199666};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC       Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC   -!- SIMILARITY: Belongs to the peptidase S45 family.
CC       {ECO:0000256|ARBA:ARBA00006586}.
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DR   EMBL; FOPP01000003; SFG92376.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I2VX73; -.
DR   STRING; 414048.SAMN04489864_103248; -.
DR   OrthoDB; 9759796at2; -.
DR   Proteomes; UP000199666; Unassembled WGS sequence.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   CDD; cd03747; Ntn_PGA_like; 1.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR   Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR   PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199666};
KW   Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT   ACT_SITE        276
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT   BINDING         207
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         348
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         351
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ   SEQUENCE   810 AA;  92213 MW;  7AC78B95DEC9B05F CRC64;
     MNRVKAFICV LIPILLAYTL NTKFGSTPPL LKFLNPFTGF WQNAEGNRQT TKHKIALKGA
     KAEINIVFDD RMIPHVFAQN DHDVYYAQGY VTAMHRLWQM DFQTRFAAGR ISEIVGEKAI
     EVDRYQRRMG MVYGAENSLK GMMEDPKSKE MILAYTAGIN AYIHSLSRAN YPIEYKILDF
     KPEDWTPIKC ALLLKQMSAV LAMGSDEFYM TNILKKFGPE VTKDLFPDYP FKEDPIIPVG
     TKWDFKPLSI PDVPKLVTEI MGSTVRTKEK IEGIGSNNWA LSGAKTASGF PLLANDPHLD
     LSLPSIWYQI QLHAPGLNTY GVSLPGAPGV TIGFNKDIAW GVTNVAADVL DFYQIKFKDQ
     THDQYWYDNA WKKTRKRLEK IQIRGAKTLI DTVYYTHQGP VVYFQKPSYG KASNVPIGSA
     LRWIAHDKSN ELMTFYILNR GKNYHDYRKA LTYYTAPAQN FIFASNKNDI AITPNGKFPI
     KWKNQGKFVL DGSNPANDWQ GWIPASQNPT VKNPSRNFVS SANQSSTDPN YPYYINWEFS
     PYERGKRIND RLTVMNKANV DSMRNLQMDS YSITAQNLLP TILSLVNQAQ LNATQKEAFN
     LSAKWNKYFE AKEIAASIFD LWTKRLFYNI WDDEFNIKNI PMRYPSRDRS IELIQNEPNS
     TWFDNVKTKE KETLRDLVNE AFKFTCDSLE RKYGPIGKNW EWGNVKNTNV PHLAKIPGFG
     TKTLNIGGGK STVNAMSEAN GPSWRMVVEL GKTPKGHGVY PGGQSGNPGS PFYDNMVDTW
     AQGKLYDLFF MQSEDDKSGK IISRLKISKK
//