ID A0A1I2XXJ7_9PROT Unreviewed; 243 AA.
AC A0A1I2XXJ7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Dual-specificity RNA pseudouridine synthase RluF {ECO:0000256|ARBA:ARBA00039989};
DE EC=5.4.99.21 {ECO:0000256|ARBA:ARBA00038922};
DE AltName: Full=23S rRNA pseudouridine(2604) synthase {ECO:0000256|ARBA:ARBA00041697};
DE AltName: Full=Ribosomal large subunit pseudouridine synthase F {ECO:0000256|ARBA:ARBA00042890};
DE AltName: Full=rRNA pseudouridylate synthase F {ECO:0000256|ARBA:ARBA00042843};
DE AltName: Full=rRNA-uridine isomerase F {ECO:0000256|ARBA:ARBA00043147};
DE AltName: Full=tRNA(Tyr) pseudouridine(35) synthase {ECO:0000256|ARBA:ARBA00041420};
GN ORFNames=SAMN05216299_10238 {ECO:0000313|EMBL:SFH18072.1};
OS Nitrosospira sp. Nsp14.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosospira.
OX NCBI_TaxID=1855333 {ECO:0000313|EMBL:SFH18072.1, ECO:0000313|Proteomes:UP000198772};
RN [1] {ECO:0000313|EMBL:SFH18072.1, ECO:0000313|Proteomes:UP000198772}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nsp14 {ECO:0000313|EMBL:SFH18072.1,
RC ECO:0000313|Proteomes:UP000198772};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA;
CC Xref=Rhea:RHEA:38875, Rhea:RHEA-COMP:10093, Rhea:RHEA-COMP:10094,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.21;
CC Evidence={ECO:0000256|ARBA:ARBA00036535};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr);
CC Xref=Rhea:RHEA:60556, Rhea:RHEA-COMP:15607, Rhea:RHEA-COMP:15608,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|ARBA:ARBA00036390};
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DR EMBL; FOPV01000002; SFH18072.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I2XXJ7; -.
DR STRING; 1855333.SAMN05216299_10238; -.
DR OrthoDB; 9807213at2; -.
DR Proteomes; UP000198772; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02555; PSSA_1; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47683:SF2; S4 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS50889; S4; 1.
PE 4: Predicted;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000198772};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 4..61
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
SQ SEQUENCE 243 AA; 27417 MW; F260463FBFECB560 CRC64;
MEKIRLSKLM SQQGLCSRRE ADNFIERGWV RVDGERITEL GTKIHPAQRI TLDKAAQAQQ
LEQVTLLLNK PIGYVSGQPE PGYRPAVSLV NHGSRFEGDR GPQRFNPMHL RGLAPAGRLD
IDSSGLLVFT QDGRIARQLI GEDSEVEKEY LVRVEGRLPL KGLALLNHGL SLDGEVLKPA
SVVWQNKDQL RFILREGKKR QIRRMCELVG LNVTGLKRVR IGKVRLGELP LGKWRYLGPD
ETF
//
