ID   A0A1I3IMJ1_9PLAN        Unreviewed;       307 AA.
AC   A0A1I3IMJ1;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=N-acetylneuraminate lyase {ECO:0000313|EMBL:SFI49194.1};
GN   ORFNames=SAMN05421753_109187 {ECO:0000313|EMBL:SFI49194.1};
OS   Planctomicrobium piriforme.
OC   Bacteria; Planctomycetota; Planctomycetia; Planctomycetales;
OC   Planctomycetaceae; Planctomicrobium.
OX   NCBI_TaxID=1576369 {ECO:0000313|EMBL:SFI49194.1, ECO:0000313|Proteomes:UP000199518};
RN   [1] {ECO:0000313|Proteomes:UP000199518}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 26348 {ECO:0000313|Proteomes:UP000199518};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the DapA family.
CC       {ECO:0000256|PIRNR:PIRNR001365}.
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DR   EMBL; FOQD01000009; SFI49194.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I3IMJ1; -.
DR   STRING; 1576369.SAMN05421753_109187; -.
DR   Proteomes; UP000199518; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR12128; DIHYDRODIPICOLINATE SYNTHASE; 1.
DR   PANTHER; PTHR12128:SF21; N-ACETYLNEURAMINATE LYASE; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
PE   3: Inferred from homology;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001365};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199518}.
FT   ACT_SITE        141
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT   ACT_SITE        171
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT   BINDING         50
FT                   /ligand="pyruvate"
FT                   /ligand_id="ChEBI:CHEBI:15361"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
FT   BINDING         213
FT                   /ligand="pyruvate"
FT                   /ligand_id="ChEBI:CHEBI:15361"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
SQ   SEQUENCE   307 AA;  32771 MW;  AFD3B5C97F592156 CRC64;
     MSAASRFQGL IAATHAPFHD DGSVNLEVIP KQADGLIQAG VAAAFVCGST GEAHSLTVPE
     RQAITRRWVE VARGTPLQVI AHVGHNCQQD AAALASAAQR DGVCAISALA PSYFKPVDVA
     ELIRFLKPVA AGAGELPFYF YDIPGMTGVS LSMTQFLEQA PAEIPNLTGI KYTNPDLVQF
     QECLAFGGGQ FDILFGTDEC LLAGLTFGAR GAVGSSYNFA APIYRRLIAA FEKKDFATAR
     AEQLVSVRLI RLLAKFGYFA AAKSVMTLLG VPVGTVRSPL RRLSADDHKS LERQLKDSDL
     WGAIQPS
//