ID A0A1I3LUT0_9PSEU Unreviewed; 1188 AA.
AC A0A1I3LUT0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=SAMN05421835_10246 {ECO:0000313|EMBL:SFI88265.1};
OS Amycolatopsis sacchari.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=115433 {ECO:0000313|EMBL:SFI88265.1, ECO:0000313|Proteomes:UP000199025};
RN [1] {ECO:0000313|EMBL:SFI88265.1, ECO:0000313|Proteomes:UP000199025}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44468 {ECO:0000313|EMBL:SFI88265.1,
RC ECO:0000313|Proteomes:UP000199025};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; FORP01000002; SFI88265.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I3LUT0; -.
DR STRING; 115433.SAMN05421835_10246; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000199025; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000199025};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1188 AA; 131100 MW; 65F192FCD94E38C3 CRC64;
MSNDSFVHLH VHTEYSMLDG AAKIAPLFAE AKRLEMPAVG MTDHGNMYGA DQFYQEAKKA
GLKPIIGIEA YIAPESRFTK KPVFWGQASQ RGSDEFGEGG DVSGGGAYTH MTMLAQNATG
LRNLFKLSSL ASIQGYYRKP RMDRELIAEN ASGIIATTGC PSGEVQTRLR LGQKREAIQA
ASDYKDIFGA DNFFLELMDH GLPIERSVRE GLLEIGKLLD LKPLATNDSH YVTKDQADSH
SALLCVQSGK TLNDPNRFKF DGDGYYLKSA QEMREYWDSE VPGAADTTLL IAERVESYED
VYTHKDRMPV FPVPEGHDEA SWLREEVERG LKWRFPDGAP EGYRERIEME LDVIIGKGFP
AYFLVVADLI NHARKVGIRV GPGRGSAAGS LVAYVLGITN LDPIPQKLLF ERFLNPERVS
MPDIDIDFDD RRRGEMIRYA TEKYGADKVA QVITFGTIKT KAAIKDSARV HFGQPGYAIA
DKISKALPPP IMAKDIPLSG IVDPQHERYG EAAEVRTLIE TDEEVSTIFT TARGLEGLIR
NAGVHACAVI MSSEPLMDAI PLWQRDDGSI ITGWDYPSCE AIGLLKMDFL GLRNLTVIGD
AIDNIKANRG IDIDLDTLGV DDPETYKLLS RGDTLGVFQL DGGPMRDLLR RMQPTVFDDI
VAVGALYRPG PMGMNAHNDY ADRKNGRQQV KPIHPELEEP LKEILADTYG LIVYQEQIMH
IGQKVAGYSM GRADVLRRAM GKKKKEVLDK EFEGFHSGMK ENGFSDEAIK ALWDTILPFA
GYAFNKSHAA AYGLVSYWTA YLKANYPAEY MAALLTSVGD NKDKSAVYLS ECRRLGIKVL
PPDVNESGPR FAAVGDAIRF GMGAVRNVGA NVVDSIIKTR EEKGKYSSFT DFLDKSELVA
CNKRVLESLI KAGAFDSLGN TRLSMIQVHE DAVDAVVPLK RQEAMGQFDL FGSADDAGEA
APSSSPLAHL KFSEEEWPRK QLLAYEREML GLYVSAHPLD GAERILRKHA PRPIAAILDN
PPKEGELVVS GLITSLERRV NKKGEPWAIC TVEDMDASLE VLFFPKSYAM FAADLVEDNA
VLVKGRVNWR EDKMSVFGGG LVPLDLSEVG NGEDEPPLML HCSAEKLDKS VVGELKSTLL
AHRGETPVRL KLIGRQRETV FALYDYPVKV TSMLMGELKG IPGITAGT
//
