ID A0A1I4AMG4_9PROT Unreviewed; 460 AA.
AC A0A1I4AMG4;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Small-conductance mechanosensitive channel {ECO:0000256|RuleBase:RU369025};
GN ORFNames=SAMN05216302_100976 {ECO:0000313|EMBL:SFK56876.1};
OS Nitrosomonas aestuarii.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=52441 {ECO:0000313|EMBL:SFK56876.1, ECO:0000313|Proteomes:UP000199533};
RN [1] {ECO:0000313|Proteomes:UP000199533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nm69 {ECO:0000313|Proteomes:UP000199533};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC of osmotic pressure changes within the cell, opening in response to
CC stretch forces in the membrane lipid bilayer, without the need for
CC other proteins. Contributes to normal resistance to hypoosmotic shock.
CC Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC preference for anions. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBUNIT: Homoheptamer. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU369025}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU369025}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|ARBA:ARBA00008017, ECO:0000256|RuleBase:RU369025}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU369025}.
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DR EMBL; FOSP01000009; SFK56876.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I4AMG4; -.
DR STRING; 52441.SAMN05216302_100976; -.
DR OrthoDB; 9793781at2; -.
DR Proteomes; UP000199533; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008381; F:mechanosensitive monoatomic ion channel activity; IEA:InterPro.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 3.30.1340.30; -; 1.
DR Gene3D; 3.30.70.100; -; 1.
DR InterPro; IPR007055; BON_dom.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR049278; MS_channel_C.
DR InterPro; IPR045275; MscS_archaea/bacteria_type.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR011066; MscS_channel_C_sf.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR PANTHER; PTHR30221; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR PANTHER; PTHR30221:SF1; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR Pfam; PF04972; BON; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR Pfam; PF21082; MS_channel_3rd; 1.
DR SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS50914; BON; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|RuleBase:RU369025};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Ion channel {ECO:0000256|RuleBase:RU369025};
KW Ion transport {ECO:0000256|RuleBase:RU369025};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU369025};
KW Reference proteome {ECO:0000313|Proteomes:UP000199533};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU369025};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU369025}; Transport {ECO:0000256|RuleBase:RU369025}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..460
FT /note="Small-conductance mechanosensitive channel"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011733512"
FT TRANSMEM 142..163
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 175..198
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 204..226
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT DOMAIN 52..118
FT /note="BON"
FT /evidence="ECO:0000259|PROSITE:PS50914"
SQ SEQUENCE 460 AA; 51052 MW; DC1EE026C34DA1C4 CRC64;
MRKSFTAFVL WMTLVVSPSY ADQLTDLIAG DTESQTTPGA DKVIDVNSSA QDDKKIQRRL
QKIFSEMDNL ENIKILTSSG IVTLQGEVNS APAEKKAIQL SQQVEGVVEV ESELVITHDL
KERLETTWQK LAKAAKALIT GFPVFLLAII AFTLTVIFGR WLSHRQSLYR RIAPNYFIAS
LLGQITRLLF ILLGLVIALS LLDLTALLGS ILGAAGIIGL AVGFAVRDTV ENYIASILLS
LRNPFEVNDL VNIDGNEGNV VRLTTRATIL LSPDGNHIRI PNSIVFKAVI INYTRNAERR
FQFDAGIDAD QDLQIAQSLA LQTLGSIEGV LAEPKPMVII EELGNSSIVL RIYAWTDQDK
FDFLKVRSET IRQIKLAFDQ ANIVMPEPIY QIKIGNETGI DLKQKDNADQ ERVYPAHKQP
RIITHATIDI STDNTIEKKV VEEHEHGDTE NLLGKNASQE
//
