ID   A0A1I4H025_9PROT        Unreviewed;       700 AA.
AC   A0A1I4H025;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   RecName: Full=transketolase {ECO:0000256|ARBA:ARBA00013152};
DE            EC=2.2.1.1 {ECO:0000256|ARBA:ARBA00013152};
GN   ORFNames=SAMN05216302_10661 {ECO:0000313|EMBL:SFL35023.1};
OS   Nitrosomonas aestuarii.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=52441 {ECO:0000313|EMBL:SFL35023.1, ECO:0000313|Proteomes:UP000199533};
RN   [1] {ECO:0000313|Proteomes:UP000199533}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nm69 {ECO:0000313|Proteomes:UP000199533};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate =
CC         aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate;
CC         Xref=Rhea:RHEA:10508, ChEBI:CHEBI:57483, ChEBI:CHEBI:57737,
CC         ChEBI:CHEBI:58273, ChEBI:CHEBI:59776; EC=2.2.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001027};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- COFACTOR:
CC       Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC         Evidence={ECO:0000256|ARBA:ARBA00001964};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC   -!- SIMILARITY: Belongs to the transketolase family.
CC       {ECO:0000256|ARBA:ARBA00007131}.
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DR   EMBL; FOSP01000066; SFL35023.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I4H025; -.
DR   STRING; 52441.SAMN05216302_10661; -.
DR   OrthoDB; 8732661at2; -.
DR   Proteomes; UP000199533; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004802; F:transketolase activity; IEA:UniProtKB-EC.
DR   CDD; cd07033; TPP_PYR_DXS_TK_like; 1.
DR   CDD; cd02012; TPP_TK; 1.
DR   Gene3D; 3.40.50.920; -; 1.
DR   Gene3D; 3.40.50.970; -; 2.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR009014; Transketo_C/PFOR_II.
DR   InterPro; IPR005475; Transketolase-like_Pyr-bd.
DR   InterPro; IPR005478; Transketolase_bac-like.
DR   InterPro; IPR020826; Transketolase_BS.
DR   InterPro; IPR033248; Transketolase_C.
DR   InterPro; IPR033247; Transketolase_fam.
DR   InterPro; IPR005474; Transketolase_N.
DR   NCBIfam; TIGR00232; tktlase_bact; 1.
DR   PANTHER; PTHR43522; TRANSKETOLASE; 1.
DR   PANTHER; PTHR43522:SF2; TRANSKETOLASE 1-RELATED; 1.
DR   Pfam; PF02779; Transket_pyr; 1.
DR   Pfam; PF02780; Transketolase_C; 1.
DR   Pfam; PF00456; Transketolase_N; 1.
DR   SMART; SM00861; Transket_pyr; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR   SUPFAM; SSF52922; TK C-terminal domain-like; 1.
DR   PROSITE; PS00802; TRANSKETOLASE_2; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000199533};
KW   Thiamine pyrophosphate {ECO:0000256|ARBA:ARBA00023052};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          370..542
FT                   /note="Transketolase-like pyrimidine-binding"
FT                   /evidence="ECO:0000259|SMART:SM00861"
SQ   SEQUENCE   700 AA;  77289 MW;  669066AF05119D75 CRC64;
     MTNVELDQLS INTIRTLSID AVQEANSGHP GTAMALAPLV YTIWNRVLRF DPQDPIWPNR
     DRFVLSNGHA SMLLWSLLHL TGTRAVNADY ERLEEPSVTL EDIRRFRQLG SKAPGHPEYH
     WVSGVETTTG PLGQGIATSV GMAIAEKWLA TRYNKPAFNI FDYNIYSICG DGCLMEGVSS
     EAASLAGHLA LDNLCWIYDH NHITIEGSTQ ITFTEDVAAR FLSYRWNVLR LGDANNIDQI
     DCALQEFHKT KDRPTLIILD SHIGYGSPHR HDTAAAHGEP LGPDEVRSTK RAYGWPEDVQ
     FRVPEGVREH FSAGIGIRGA SACREWTTLF ANYRAKYPEL ATEIDQMQRR ELPVNWDCNL
     PVFPEDPEGL AGREVSGKVL NILARNIPWL LGGSADLGSS NKTLLTYQGA EDFQAATPGG
     KNLHFGIREH AMGAIVNGLS LSKLRPYGAT FFIFSDYARP AIRLSALMEL PAIFIFTHDA
     MGDGEDGPTH QPVEHLASLR AIPGLTLLRP GDANEVVEAY RVIMQLRHRP AILVLSRQAL
     PTLNRRKYAS ASGVARGAYV LADALGGKPD IILIATGSEI SLAVQAHEKL VSEGICSRVV
     SMPSWDIFEH QTQEYRDSVL PPMVKARVAI EQASTFGWER YVGNSGRVIG MQTFGASAPL
     KELQKEFGFE PERVVLVAKE ILGKAYSRGN IKKRSFSAPC
//