ID   A0A1I4HJY9_9PORP        Unreviewed;       495 AA.
AC   A0A1I4HJY9;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=23S rRNA (Uracil1939-C5)-methyltransferase {ECO:0000313|EMBL:SFL42628.1};
GN   ORFNames=SAMN05216357_12243 {ECO:0000313|EMBL:SFL42628.1};
OS   Porphyromonadaceae bacterium KH3CP3RA.
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Porphyromonadaceae.
OX   NCBI_TaxID=1855396 {ECO:0000313|EMBL:SFL42628.1, ECO:0000313|Proteomes:UP000198708};
RN   [1] {ECO:0000313|EMBL:SFL42628.1, ECO:0000313|Proteomes:UP000198708}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KH3CP3RA {ECO:0000313|EMBL:SFL42628.1,
RC   ECO:0000313|Proteomes:UP000198708};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; FOSU01000022; SFL42628.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I4HJY9; -.
DR   Proteomes; UP000198708; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   NCBIfam; TIGR00479; rumA; 1.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          31..89
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        453
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        453
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         327
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         356
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         377
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         426
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   495 AA;  56393 MW;  50D2726C3475B6C6 CRC64;
     MRLGTAQTSL ALLSAFTIFV PQNGGKMAKK RKQLPLLENI LITDVAAEGK AIAKVDGRAV
     FIPFAVPGDV VDIQLTRKKN SFAEGRVIRF EKYSENRTEP FCIHFGVCGG CKWQMLPYER
     QLKHKQQQVT DNLERIGKID LPDFNPILGA PHATFYRNKL EFTFSNKKWL TEAQIASNET
     FDNMNALGFH IPGMFDKVLN IDKCWLQDDL SNRIRNAVRE FCLKNDYSFF DLRNQVGLMR
     TLIIRNSSIG EWMVIVVFYE DDPEKREKLM NFIAGEFPQI TSLLYIVNQK ANDTITDQEV
     IVWRGRDHIY EEMEGLKFKI GPKSFFQTNS EQAYNLYKVA RDFAQLSGKE TVYDLYTGTG
     SIANFVARNA RKVVGIEYVP EAIYDAHENS RLNGIGNTLF FAGDMKDVLT ADFISEHGCP
     DVIITDPPRA GMHNDVIDAI LLAEPVRIVY VSCNPATQAR DLSLLDGKYR VTRVQPVDMF
     PHTHHVENVV LLEKR
//