ID A0A1I4IJ10_9BACI Unreviewed; 742 AA.
AC A0A1I4IJ10;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=SAMN04487943_102119 {ECO:0000313|EMBL:SFL53801.1};
OS Gracilibacillus orientalis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Gracilibacillus.
OX NCBI_TaxID=334253 {ECO:0000313|EMBL:SFL53801.1, ECO:0000313|Proteomes:UP000198565};
RN [1] {ECO:0000313|EMBL:SFL53801.1, ECO:0000313|Proteomes:UP000198565}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.4250 {ECO:0000313|EMBL:SFL53801.1,
RC ECO:0000313|Proteomes:UP000198565};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
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DR EMBL; FOTR01000002; SFL53801.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I4IJ10; -.
DR STRING; 334253.SAMN04487943_102119; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000198565; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536}.
FT DOMAIN 31..286
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 650..738
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 479
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 549
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT BINDING 200
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 367..368
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 444
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 477..481
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 527
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 549
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
SQ SEQUENCE 742 AA; 86444 MW; 4289F6ED85E84AE7 CRC64;
MPIQVNEEQL EFHLYNDNVS YIFRVLEKSH QLEHLYYGKR IHHRKSFAHL IEREIRPSTN
MFEGDHTSSM EHIKQEYPSY GSTDFRYPAH TILRQDGSHI TDFTYDSFRV INGKKPLTDL
PATYVENEDE ADTLEIELVD DILQCRLILS YTIYQNRNVI TRHAKFINEG LESYYLDQAM
SMNIDLPDDQ FDMLHLQGAW AREGHVETKR LSKGIQSIYS NRGGSSHVFN PFFALKRWNT
DENQGEVYGF SLVYSGNFLA QIEVDTYNVS RVNMGINPYR FKWKLASGGS FQTPECVMVY
SDQGLNGMSQ TFHKLYRERL VRGYWRDKVR PILINNWEAT YFDFNEEKVL NIAKTAQELG
IELFVLDDGW FGKRDDDTSS LGDWFEYEEK LPNGIDGLSE KVEALDMRFG LWFEPEMVCK
DTKLFEKHPD WIISTPERRA SHGRNQFVLD FTRTEVVDYI FDLMDKIISK SNISYIKWDM
NRHITEAFSQ NLPADQQGEV FHRYILGVYD LYERLIKKYP EILFESCAGG GARFDPGMLY
YAPQTWTSDD TDAVERLKIQ YGTSMVYPLS AIGSHVSAIP NHQVGRLASL DTRAAVAYFG
TFGYELDITQ LTEEEKQKVK EQVAFFKEKR SLIRDGEFYR LSSPFEYNET TWMVVNEEKS
EAIVGYYKVL AKPNEAYNRI KLTGLDPEKL YEINGENSYY GSELMNVGIV LAGNFIDRAD
EYWGQEHPGD YQSRLFILKA LS
//