ID A0A1I4S189_ECTMO Unreviewed; 833 AA.
AC A0A1I4S189;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=CRISPR-associated endonuclease/helicase Cas3 {ECO:0000313|EMBL:SFM58040.1};
GN ORFNames=SAMN05421721_11156 {ECO:0000313|EMBL:SFM58040.1};
OS Ectothiorhodospira mobilis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Ectothiorhodospira.
OX NCBI_TaxID=195064 {ECO:0000313|EMBL:SFM58040.1, ECO:0000313|Proteomes:UP000199556};
RN [1] {ECO:0000313|EMBL:SFM58040.1, ECO:0000313|Proteomes:UP000199556}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 4180 {ECO:0000313|EMBL:SFM58040.1,
RC ECO:0000313|Proteomes:UP000199556};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
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DR EMBL; FOUO01000011; SFM58040.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I4S189; -.
DR STRING; 195064.SAMN05421721_11156; -.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000199556; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR PANTHER; PTHR47959; ATP-DEPENDENT RNA HELICASE RHLE-RELATED; 1.
DR PANTHER; PTHR47959:SF16; CRISPR-ASSOCIATED NUCLEASE_HELICASE CAS3-RELATED; 1.
DR Pfam; PF18019; Cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Endonuclease {ECO:0000313|EMBL:SFM58040.1};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:SFM58040.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000199556}.
FT DOMAIN 278..435
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 606..825
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT REGION 660..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 678..695
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 833 AA; 92348 MW; BB81910018277D30 CRC64;
MSFESFFAAA MGEGCSPFPY QKRLTEGPWP DCVEIPTGLG KTAGIALAWA YRRGVRADGE
RKAPDPDTPR RLIWCLPMRV LVEQTARNIR EWLQRLQVLG DAGEPGRIAV HMLMGGEADL
RRGAWAAYPE QDAVLVGTQD MLLSRALMRG YAMSRYQWPV HYALLHNDAL WVLDEAQLMG
PALPTTTQLE AFRRMLDTAA PARSVWVSAT LHTDWLDTVD FRPYQRSGLQ HLQLEDADRR
SPAVAARIQA AKRLDLAETR LLASNGKISK GESAYTRDLA REVIAAHRDG EQTLVILNRV
DRAQALFDAL AGTDAQRLLL HARFRPAERA AIESQLQEPP GESGRIIIAT QAVEAGVDLD
SRVLFTELAP WASLVQRFGR CNRAGRFEDA RIYWIDLDEN TGDLALPYEA ISLARAREQL
RAAASAAPAD LPPVTEAPPT DQVLRRRDLL DLFNTDPDLS GFDVDIAPYI RDTGTPPVQV
FWRAVDDDEA AAEQGAPSRE ELCSVSVGQL RNHLSRKLPT PRRRKLGREV RFAWRLDPLA
GRGRGGRWRR IRADEVRPGQ TILLAAADGG YDPHRGFVAG DAEPVAVIPQ KNGVDPEGYG
SDVLSHMGRR VPLPEHLADV AAAMADLGHS LECPQKERTL LELAARWHDV GKAHEAFQRG
IGFEPGDPDG PWAKSDQSGR PDYHRLDGQG RREDRPGFRH ELASALAWLE AGGGGLSPSE
RNRVAYLIAA HHGRVRLGLR ALPDEMEPPE PGILYARGIW DGDSLPALNL HGLRLEPVTL
RLDLMRLGNG PQGPSWSERT QRLLNELGPF RLAWEEALVR IADWRASARE AGE
//
