ID A0A1I5ARG6_9CLOT Unreviewed; 1171 AA.
AC A0A1I5ARG6;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=SAMN04488695_103147 {ECO:0000313|EMBL:SFN64980.1};
OS Proteiniclasticum ruminis.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Proteiniclasticum.
OX NCBI_TaxID=398199 {ECO:0000313|EMBL:SFN64980.1, ECO:0000313|Proteomes:UP000181899};
RN [1] {ECO:0000313|EMBL:SFN64980.1, ECO:0000313|Proteomes:UP000181899}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ML2 {ECO:0000313|EMBL:SFN64980.1,
RC ECO:0000313|Proteomes:UP000181899};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; FOVK01000003; SFN64980.1; -; Genomic_DNA.
DR RefSeq; WP_074911760.1; NZ_FOVK01000003.1.
DR AlphaFoldDB; A0A1I5ARG6; -.
DR STRING; 398199.SAMN05421804_103290; -.
DR eggNOG; COG1197; Bacteria.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000181899; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000181899}.
FT DOMAIN 645..806
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 815..981
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1171 AA; 135633 MW; 8E7B24AAD57F3ADA CRC64;
MRLEGLRQRL YEDGSFKELR KSIKEKKFPV GVFGVSESAR AYLISTLFGK EKDSIFIFTS
KDMDAKNLYE DLLLYENEVY YFPAKDLVFY NIDAISGDLR WERLKVMKAL QNGKKKIVVT
TIDALTAKYA PYEYFRAYHF SLEKGMEINL KEVGKKLQSA GYVRVELVEG KGEYALRGGI
LDVFPPDSAQ PYRVELFGDE IDSIRTFHPE NQRSIEQVPS FEIFPAKELI LTEERMKEGA
ERIEKEFQDI LKDKKKRKGY DNEAFEKLKG HVSSNLEYLK EGVFFENIDA YLPFFFEKPS
SFFDFMGEAK IIVDDTAHSL GKLESVYQEF QEDYDAFLHR GEIMPSQGSL MLEKDLLLET
LKNKDVITLN QFQKTERFLP PRSLVNFNQI TIYNYQGQLD LLIDDIKDRK RRGYKVVILA
GTRPRGERLV DILRDKDIES VYKDTLSTLD YSDVIITFGN QLRGFEFPDI RLSLISDKEV
FGEAKRKKRK SPKKGKGLSK IQSFTELRKG DYVVHVNHGI GVFLGIKQLE SSGLVRDYIE
ISYDKNDKLF IPVEQLDMIQ RYIGTEGKQP KINKLGGQEW SKAKAKVKRS VDEIAEDLVK
LYAEREKVKG YPFTPDTEWQ RQFEDEFAYD ETEDQLRSSE EIKRDMERER VMDRLLCGDV
GYGKTEVAMR AAFKAVMDSK QVAVLVPTTI LAEQHYHSFR NRFKGFPIKI DMISRFRGPK
EQKETMRRVK EGNVDIIIGT HKILSKDLQF KDLGLLIVDE EQRFGVAHKE KLKSAKKNVD
VLTLSATPIP RTLHMSLSGI RDISSIETPP EERYPIQTYV VEFNEQLVRD AILREKARSG
QVYFVHNRVD DIHVMHSYLQ KLVPEVIFDV AHGQMNEREL ENAMKNFMDG HTDVLISTTI
IETGMDIQNV NTIIVDHADK LGLSQLYQLR GRVGRTNRIA YAYLTYKKDK VLTEIAEKRL
RALKDFTELG SGFKIAMRDL EIRGAGNLMG KAQHGQMSVV GYDLYVKMLD TAIKMLTGEI
QEEKVDTTID IRVDAYIPSS YISDEMHKIE VYKRIASMES EEDYEDIKEE LLDRFSDIPD
SLYNLMDIAR LKFYANRAGV LELKDRMKDI LVTFVSSEKL TEEKVSHLMK KYSKRVEFIR
STPGFSVKNL EKDRDETIMF LTKMMKELSA L
//
