ID A0A1I5BZW3_9ACTN Unreviewed; 860 AA.
AC A0A1I5BZW3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=SAMN04489713_10391 {ECO:0000313|EMBL:SFN80300.1};
OS Actinomadura madurae.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Thermomonosporaceae; Actinomadura.
OX NCBI_TaxID=1993 {ECO:0000313|EMBL:SFN80300.1, ECO:0000313|Proteomes:UP000183413};
RN [1] {ECO:0000313|EMBL:SFN80300.1, ECO:0000313|Proteomes:UP000183413}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 43067 {ECO:0000313|EMBL:SFN80300.1,
RC ECO:0000313|Proteomes:UP000183413};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR EMBL; FOVH01000003; SFN80300.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I5BZW3; -.
DR STRING; 1993.SAMN04489713_10391; -.
DR eggNOG; COG2352; Bacteria.
DR InParanoid; A0A1I5BZW3; -.
DR Proteomes; UP000183413; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 2.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:SFN80300.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000183413}.
FT ACT_SITE 134
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 523
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ SEQUENCE 860 AA; 94649 MW; 867DA13A2B4C257E CRC64;
MPEPLRRDVR LLGAMLGDSL VEYGGTGLLD DVERLRRAVI AARRGETGIE EVAALVDGWS
LERAGQVARA FTVYFHLTNL AEEHHRIRTL RERDTGDTVP GSAAAAIEEI RASGDGRLDA
IIEGLEFRPV FTAHPTEARR RAVVTAIQRI SDLLARLNAG PGASERQETE RSLREEIDLL
WRTALRRGTQ IDPLDEVRTA MAAFDETIFR VVPAVYRALD RALDGERTGT RPPKARPYLR
FGSWIGGDRD GNPYVTAQVT RDAVMIQADH VLRALENACA RVGRELTVHE STTPPSKDLL
NALAAARTAH PSRLREIAER SPGEEHRQLL LYAAARIAAT RERDADLAYA SPDELLADLR
TVQDSLAGAG ATRQAYGRVQ QLVWQVETFG FHLAELEIRQ HSELHEKALA ELDGDHSEMT
DEILDTLRTV AWIQNRFGVR ACHRYIVSFT RSAADIANVH ELAASLGDGA PVLDVIPLFE
TGEDLERAPS VLDGMLEIPA VRRRLDETGR RLEVMLGYSD SAKQLGPTSA TLHLYDTQEA
LTAWAARHDI TLTLFHGRGG SLGRGGGPAN RAILAQAPGS VAGRFKVTEQ GEVIFARYGH
AEIAKRHVEQ VTSAVLLAST DPVQDRAREA AARFRPLADK IGEAAEAAFR SLVETDGFAA
WFARVSPLEE ISELRIGSRP ARRKAARGLE DLRAIPWVFA WTQTRVNLPG WYGLGSGLAA
VSDDGRDLTG LREAYEAWPL FNTLLDNAEM SLAKSDRAIA ERYLALGERP ELSAAVLAEY
DRTHRLVLAV TGHDRLLENR RVLSRAVELR NPYVDALSHL QLRALKALRA GVDDEDERTH
LESLLLLSVN GVAAGLQNTG
//
