ID A0A1I5G6S0_9BURK Unreviewed; 412 AA.
AC A0A1I5G6S0;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=carboxymethylenebutenolidase {ECO:0000256|ARBA:ARBA00044062};
DE EC=3.1.1.45 {ECO:0000256|ARBA:ARBA00044062};
GN ORFNames=SAMN05443579_102376 {ECO:0000313|EMBL:SFO31710.1};
OS Variovorax sp. PDC80.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=1882827 {ECO:0000313|EMBL:SFO31710.1, ECO:0000313|Proteomes:UP000199369};
RN [1] {ECO:0000313|Proteomes:UP000199369}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PDC80 {ECO:0000313|Proteomes:UP000199369};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC 2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC Evidence={ECO:0000256|ARBA:ARBA00043789};
CC -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC {ECO:0000256|ARBA:ARBA00008456}.
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DR EMBL; FOWG01000002; SFO31710.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I5G6S0; -.
DR OrthoDB; 62567at2; -.
DR Proteomes; UP000199369; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR Gene3D; 3.10.450.50; -; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002925; Dienelactn_hydro.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR037401; SnoaL-like.
DR PANTHER; PTHR46623; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR PANTHER; PTHR46623:SF11; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR Pfam; PF01738; DLH; 1.
DR Pfam; PF12680; SnoaL_2; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF54427; NTF2-like; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000199369}.
FT DOMAIN 20..233
FT /note="Dienelactone hydrolase"
FT /evidence="ECO:0000259|Pfam:PF01738"
FT DOMAIN 253..359
FT /note="SnoaL-like"
FT /evidence="ECO:0000259|Pfam:PF12680"
SQ SEQUENCE 412 AA; 45448 MW; F9C9C80117AB2C70 CRC64;
MTAQKGRWLQ VPAIDGSGSF RAYLATPASG KGPGIVLAQE IFGVNATMRQ VADYYAEEGY
VVLAPDLFWR QQPEVELGYA PDDWQKAFAL YKGFDEDKGT EDVAASLALL RSLPEQQGKA
GVLGFCMGGK LAYLAACRTD ADVCVGYYGV GIEQRLEEAE RIQGRLVLHI PELDGFCPPE
ARSRIVETLG ARPGCELYVY PGVDHAFARL GGDHYHKPSA LMAHERSVAA FRREMGPHYD
FSALWDKHCE YEFGTRNVAD TMATMVAEPY VNHIPTMTGG VGHAMLSRFY QHHFVNSNPP
DTRLVPISRT VGATQIVDEM LFCFTHTCEI DWLLPGVAPT GRPVEIPLVA IVKFRGDKLY
HEHIYWDQAS VLVQVGLLDP KGLPVAGVET ARKLLDETQP SNTLMAAWSR SG
//
