ID   A0A1I5KYJ7_9PSEU        Unreviewed;       282 AA.
AC   A0A1I5KYJ7;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 13.
DE   RecName: Full=protein-S-isoprenylcysteine alpha-carbonyl methylesterase {ECO:0000256|ARBA:ARBA00038928};
DE            EC=3.1.1.n2 {ECO:0000256|ARBA:ARBA00038928};
GN   ORFNames=SAMN05421805_12867 {ECO:0000313|EMBL:SFO90139.1};
OS   Saccharopolyspora antimicrobica.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Saccharopolyspora.
OX   NCBI_TaxID=455193 {ECO:0000313|EMBL:SFO90139.1, ECO:0000313|Proteomes:UP000199398};
RN   [1] {ECO:0000313|EMBL:SFO90139.1, ECO:0000313|Proteomes:UP000199398}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CPCC 201259 {ECO:0000313|EMBL:SFO90139.1,
RC   ECO:0000313|Proteomes:UP000199398};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl
CC         ester + H2O = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine +
CC         H(+) + methanol; Xref=Rhea:RHEA:48520, Rhea:RHEA-COMP:12125,
CC         Rhea:RHEA-COMP:12126, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17790, ChEBI:CHEBI:90510, ChEBI:CHEBI:90511; EC=3.1.1.n2;
CC         Evidence={ECO:0000256|ARBA:ARBA00035789};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|ARBA:ARBA00004586}. Golgi apparatus membrane
CC       {ECO:0000256|ARBA:ARBA00004653}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004653}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Isoprenylcysteine
CC       methylesterase family. {ECO:0000256|ARBA:ARBA00038028}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FOUP01000028; SFO90139.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I5KYJ7; -.
DR   STRING; 455193.SAMN05421805_12867; -.
DR   OrthoDB; 9803828at2; -.
DR   Proteomes; UP000199398; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR049492; BD-FAE-like_dom.
DR   PANTHER; PTHR48081; AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C; 1.
DR   PANTHER; PTHR48081:SF36; ALPHA BETA-HYDROLASES SUPERFAMILY PROTEIN; 1.
DR   Pfam; PF20434; BD-FAE; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   3: Inferred from homology;
KW   Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT   DOMAIN          63..158
FT                   /note="BD-FAE-like"
FT                   /evidence="ECO:0000259|Pfam:PF20434"
SQ   SEQUENCE   282 AA;  30278 MW;  6F0597E43835E45D CRC64;
     MWDSAALDRE FSPSLLAPGF EDVLRTYAER SAEARSACPG FRTLSYGEHP DEVLDHFPAR
     EPGSPLHVFI HGGHWQELSK DESSFAALDF LARGHGFAAL NYGLAPRRSL EEIVGSAHRA
     LCWIRDNAAA LGTRPDAVHA SGSSAGAHLL AMACCLDDRA PGNRPPLASA CLISGTYDLE
     PLRHSYVNDA LGLDEQRARA LSPLHELPCR VRRLVLARGE RETGEYIRQH QLFSAAARRA
     GHQVTDVVVA GTNHFDVVFG LADPAGPLGA AVLADAGPRP AG
//