ID A0A1I5P5G2_9ACTN Unreviewed; 6570 AA.
AC A0A1I5P5G2;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Methyltransferase, FkbM family/amino acid adenylation domain-containing protein {ECO:0000313|EMBL:SFP29197.1};
GN ORFNames=SAMN04489713_1132 {ECO:0000313|EMBL:SFP29197.1};
OS Actinomadura madurae.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Thermomonosporaceae; Actinomadura.
OX NCBI_TaxID=1993 {ECO:0000313|EMBL:SFP29197.1, ECO:0000313|Proteomes:UP000183413};
RN [1] {ECO:0000313|EMBL:SFP29197.1, ECO:0000313|Proteomes:UP000183413}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 43067 {ECO:0000313|EMBL:SFP29197.1,
RC ECO:0000313|Proteomes:UP000183413};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FOVH01000013; SFP29197.1; -; Genomic_DNA.
DR STRING; 1993.SAMN04489713_1132; -.
DR eggNOG; COG1020; Bacteria.
DR InParanoid; A0A1I5P5G2; -.
DR Proteomes; UP000183413; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 4.
DR CDD; cd17646; A_NRPS_AB3403-like; 1.
DR CDD; cd19544; E-C_NRPS; 2.
DR CDD; cd19540; LCL_NRPS-like; 4.
DR Gene3D; 3.30.300.30; -; 7.
DR Gene3D; 3.40.50.980; -; 8.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 6.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 6.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR006342; FkbM_mtfrase.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 6.
DR NCBIfam; TIGR01444; fkbM_fam; 1.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF13193; AMP-binding_C; 6.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF05050; Methyltransf_21; 1.
DR Pfam; PF00550; PP-binding; 6.
DR SMART; SM00823; PKS_PP; 6.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 6.
DR SUPFAM; SSF47336; ACP-like; 6.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 12.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00455; AMP_BINDING; 6.
DR PROSITE; PS50075; CARRIER; 6.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 6.
PE 4: Predicted;
KW Methyltransferase {ECO:0000313|EMBL:SFP29197.1};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000183413};
KW Transferase {ECO:0000313|EMBL:SFP29197.1}.
FT DOMAIN 960..1035
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2314..2389
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3347..3421
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4372..4447
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5418..5492
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 6475..6550
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 6570 AA; 696617 MW; 3179E629F6340767 CRC64;
MIPLSFAQRR LWFLAELEGP GASYNLPMAL RLTGDLDRPA LEAALRDVLE RHEVLRTVFP
AVDGEPHQRI LTAEETGFDL PLVETTAGER SGVMAESARY AFDLSTEIPF RAWLFAAGPR
EHVLLMVVHH IAGDAWSTRP LARDLAAAYT ARRAGRAPEW TPLPVQYADY ALWQRELLGA
EDDPGSVLSR QVAYWRETLA GAPEELALPY DRVRPAAASH RGHRVALDLP PDVHARVRAV
ARAEGVTVFM VLQAALAVTL HRLGAGTDIP TGAAVAGRLD ESLDDLVGFF VNTLVVRADL
SGDPTFGDLL GRVRDASVGA YAHQDVPFER LVEELAPARS LARHPLFQVM LTVQNAGSAE
LSLPGLDVSG ISNAALSEER TTRFDLDVAV REAFDAEGAP AGMRGRLVAS ADVFDEDTAG
RIAEWFARVV EAVVQEPGRP VAAVDLVDGT ERDRLLAWGH GPALEAPEGL VPEMFAAQVA
RAPDAVAVAH GDDELTYAEL DVRANRLAHR LRAQGVGAES VVGLCVPRGI PTVVAILGVW
KAGAAYVPLD PEYPADRLAF MLADSGARLL VGRGDIIPGS AGVTVLDLDD PRLTETSEDP
PDVPVTPRQL AYVIYTSGST GRPKGVGVSH ASVANMATAL APALGAGPGT RVLQFASFSF
DASVLDVAVT LAAGGTLVLA SSEERSDTRA LARLIREARI STMSVVPSLL EVLDPDDVPD
LSRVVVGAEP IGSAQAAAWS DGRHLVNTYG PTEATVMVTA GPVDGTEPVP MGGPVANVSL
FVLDERLAPV PVGVPGELYI AGAQVARGYV GRPGLTGERF VACPFAAGAR MYRTGDLVRW
TPGGRLVFAG RADEQVKIRG FRVEPGEVRA VLAEHSQVAR AAVVVREDTP GDRRLVGYVV
PAGAGADPAG IRDFAASRLP DHMVPAAVVA LDALPLTANG KLDRGALPAP AYPGGGTGRA
PATAREEILC QVFAQVLGLD RVGPDDDFFR LGGHSLLATR LVSRVRTVLG AELPLRALFE
APTAAGLAAR LSETGRARVA PAPMRRPDRV PLSYAQRRLW FLAQLEGPSP TYNISMALRL
TGELDRRALE AALRDVLERH EVLRTVFPAV DGEPYQRILA VEDTGFELPV VEVAAEALPD
AVAEAGRSVF DLSAEVPVKG WLFAVEPGEH VLTLVVHHIA SDGWSGGPLA RDLNAAYAAR
VAGRAPEWAP LPVQYADYAL WQRELLGSEH DPDSLLSRQV AYWREELAGA PEELELPYDR
GRPPMASHLG YRVPLELPAE VHARLREVAH DEGVTVFMVV QAAVAVMLHR LGAGTDVPIG
SVVAGRLDEA LDDLVGFFVN TLVIRTDLSG DPTFAEVLGR VRQTGLAAFE HQDAPFERLV
EELAPVRSLA RHPLFQVMLT LQNNAPTDLD LPGVRVSAGA AQRSGTVTAK FDLEITFTEA
FDQDGAAAGM RGAVTAAADV FEAETAGRFA GWLARVVEEV VAAPDVRVGA VGLLGDAERA
VIVGEWSAGE SVPVDRSTIP ALFEAQAART PDAPAVAFEG VTVGYAELDA RANRLARRLA
ERGAGPESVV AVMVPRSVEM MVALLGILKA GAAYLPIDPA YPEDRVALLY DDARPLVTVA
ARDVPVPDGV AHVLVDDPAV PAEDDAGDAV PAGAPSPANP AYVLYTSGST GRPKAVVVEH
AAIVNRLLWM RDHYAVNAGD RILQKTPLGF DVSVWELFLP LISGAALVVA RPDGHRDPAY
LAELIQRAGV TVTHFVPSLL QDFVQEPAAR ECTTLREVIC SGEALSAHLR DRFHSVLDAG
LNNLYGPTEV AVDVTAAACP PGTGPVTIGR PVWNTRVRVL DARLGPVPPG VPGELFLAGA
QLARGYRDRP GMTAERFVAD PSGAPGERMY RTGDVVRWDR HGRIEYLGRA DDQVKIRGQR
IEPGEVEAVL TDHPGVERAA VVARPAEGGP RLVAYVVPDT DAAGPAANIA RLRRQGRLDG
QEMHELPNGM MVCARNPSST AFLYDEIFVR DEYLRAAAAL PEGAHVVDVG GHVGLFSLFV
KERRPDCRIH AFEPIPELAR MFRLNAELHD VDAVVTDCGI GSEEGVAAFT YYPDLSLLSG
RFADEREERR VLEQVVGADA ARAGLDEHAL AELLTERLRG ERVEVPMRTL SQVIRDNGIP
VIDLLKVDVE KSELDVLRGI EPEHWPIIRQ IVAEVHDIDG RLDTVVGLLR DRGFRTSVES
TDDLAGTGMY SVHAARPDAA GRPAPPPAAT APRWFDPARL GDDLRAHART RLPDAMLPAA
FVVLDALPVT VNGKLDRAAL PAPDFAAAKP GGRGPLTARE EILCQTFARI LGLEHVGPDD
DFFKLGGHSL LATRLVSRVR AVLGVEVPLR AVFEAPTAAG LAARLADADR ARPPLLPEAR
PERVPLSFAQ RRLWFLGELD GPNATFNLTT TVRLSGGLDR RALESALADV LGRHEVLRTV
FATADGEPYQ RVLGVEETGF GLATAAVTAE HLPEAVREAG AYAFDLSAEV PIKAWLFAVR
PGEHVLMLVV HHIASDGWSM GPLARDVSIA YAARRAGRAP EWAPLPVQYA DYALWQRELL
GSEGDPDSLL AEQVAYWREM LAGVPEELEL PYDRGRPPMA SHLGYRVPLE LPAEVHARVR
EVARAEGVTV FMVVQAAVAV MLHRLGAGAD LPIGSAVAGR LDEALDDLVG FFVNTLVIRT
DLSGDPTFAE VLGRSRETSL GAYEHQDVPF ERLVEELAPV RSLARHPLFQ VMLTLQNNAP
TDLDLPGVQV SAGTSQRSQS VTAKFDLEIT FTEAFDREGA AAGIRGAVTV AADVFEAETA
GRFAGWLARV VEEVVAAPDV RVGAVGLLGD AERDRLVRAW NDTAAEVPAV SVPELFEARA
ARAPDAVAVV ADGAEVSYAE LDARVDRLAG LLRSQDVGAE SVVGLCLPRG IGMVTAILAV
WKAGAAYVPL DPGDPVARLE WMVAECGARV VVGNGEVVLP GVRNLNPDEA PAAGTRTSRV
PVTPDQAAYV IYTSGSTGRP KGVVATHGGL VNLALALGPV LGAGPGVRVL QFASFGFDAS
VLDVAATLVA GGTLVVASAA ERSDPELLTR MIRRTGVGTT SVVPSLLGVL DPDGLPEPST
ILVGAEPIGR AQAQAWSRDR RLVNTYGPTE ATVMVTTGRV DGSEPVVPMG RPVANTRLFV
LDGRLRPVPV GVAGELYIAG AQITRGYAGR PGLTAERFVA CPFGGRMYRT GDLARWTADG
ELVFAGRADE QVKIRGFRVE PGEVEAVLAA HPEVARAAVV VRDERLVAYV IGDAGEDELH
ELAAARLPGY MVPSAFVSLD ALPLTANGKL DRAALPEPEY ARVAGRGPAT VQEEILCQVF
AEVLGLDAVG VRDDFFRLGG HSLLAVRLVE RLRERGVTVS VRALFESPTP EGLAAVAGPA
AVVVPENRIP AGATEITPDM LTLVDLSADE IGRVVATIDG GAANVADVYP LAPLQEGLFF
HHVMADGGND AYVMPRRLRF DSRERLDAFL AGLQRVIDRH DIYRTAFVWE GLAEPVQVVA
RRAELPVHEV ADLDAADVPA MDVGRAPLVD VHIAAEAGDS GEWRALLRLH HLVQDHTAQD
ILIEELSAIV GGHEDSLPEP LPFRDFVAQA RLGVSREEHE RYFGELLGDV EETTAPYGLA
DVLGDGSDVR RAHMPVEDEV SRRIREAARG LGVSPATVFH LAWARVLGAV SGRDDVVFGT
VLFGRMNAGA GAERVPGLFI NTLPVRVRLG GAGVGEALDG LRSQLAELLA HEHAPLSLAQ
QAAGIPGGGA LFSSILNYRH SPVRTVQGGE GISTESIREA TNYPVAMSVE DHGAGFGLTV
DAVEAVDGAA LCRLLHTCLA GLVTALEDDP GARLDALDVL GKVERDRLVG EWNATAVEVP
PVPVVEMVEA QAARTPDAVA VAAGGTEVTY AELNARANRL AHRLRARGVR PESVVGVRLP
RGIDMVTAIV GVWKAGAAYL PIDPDQPEER IAFLLADADP VLVLGTEDID ATGPDGDPGV
PVSPDQMAYV IYTSGSTGHP KGVAVTHRGL ANYVATVPAR VGFTAGRFAV LQGQATDLGN
TAVFASLASG GTLHIPDEDT VLSPAAVRAF MAEHRIDRLK AVPSHLAALG TDVLPGGSLV
LGGEAPPPEL VAELVAGDRA VFNHYGPTET TIGVATARLT PELVEAGVVP IGSPVANTRV
YVLDDRLRPV PVGVAGELYV AGVQVARCYV GRPGLTGERF VACPFAGAGE RMYRTGDLAR
WTGDGLLVFA GRADDQVKIR GFRVEPGEVR SVLARHEGVT DAAVVVRDER LVAYVAGDVA
EADLREYAAA KLPAHMVPSA VVTLEALPLT GNGKLDRAAL PAPGYATGAG REPAGVREEI
LCQVFADVLG VDGVGPDDDF FTLGGHSLSA MRLVSRVRAV LGTEMPLRSV FEAPTPAGLA
ARLFEAEPAR APLAARTRPE RVPLSYAQRR LWFIGQLEGP SPTYNIPMAL RLTGELDRRA
LEAALRDVLE RHEVLRTVFP AADGEPYQRI LDVDETGFEL SVSATAPEDL RRAVAAAGEY
AFDLSAEVPI KAWLFGVAPD EHVLVLVTHH IAQDGWSRGP LGRDLSTAYA ARCAGREPAW
TPLPVQYADY SIWQRELLGS DDDPGSPLSR QVAYWREALA GVPEELALPF DRPRPPAASH
RGHAVGIDVP AEVHARIREV ARAEGVTVFM VLQAALAVTL SRLGAGTDVP IGAAVAGRLD
GALDDLVGFF VNTLVIRSDL SGDPTFAELL ARVRETSLDA FAHQDVPFER LVEELAPARS
LGRHPLIQAM LILQNTGGTR LALPGVEVAG ASATTPKSEF DLEINATEVF DGGGAPAGVR
CSLLAAADVF EAESAARIAG VWSRMLAALV SDPVARVGAV EALDAAERDR LLRVWNDTAV
DVPALTIPEL FAAQVERAPD ADAVAADGVR ISYRELDARA NRLARLLVRH GTGPESVVAV
AMERGVDLTV ALLGVVKAGA AYLLLDADHP AERIAFMLRD ADAHTVVTTG DWTGALSGDV
ARVVVDDPAT ILELGGLDEA AAGAAPALAS AAYVIYTSGS TGVPKGVVVS HAGVASMLDG
HVRHLGVGPG SRVGQFASAA FDTFGWEWMS ALLTGATLVV IPRDRRLGAA LPKLLADERV
SHVTLPPAVL ATLDEAAVDP GVVLVVAGEA SSGEVLDRWA PGRRMFNSYG PAETTVDATL
WECDPAAGEV SIGAPVANTR VFVLDEALRP VPVGVTGELY VAGAGLARGY AGRPDLTGER
FVACPFGGPG ERMYRTGDLA RWASDGRLAF AGRADDQVKI RGHRIELGEI ESVLAGDARV
ARVAVVARED SAGDRRLVAY VVGRADEDRL RRLAAERLPD YMVPAAFVTL DELPLTTNGK
LDRAALPAPE VAAGEGRAPA TVQEELLCHA FAQILGLDHV GVEDDFFRLG GHSLLAVRLV
DLLRGRGVSV SVRALFESPT PAELAVVAAP EAIEIPPNRI PAGATEIAPD MLTLVDLSAD
EVERVVAAVE GGAANIADVY PLAPLQEGLF FHHLLQADGG RDVYASPNVL GFDSRERRDG
ILAALQRVID RHDIYRTAIV WEGVREPVQV VVRRAVLPVR EVVLDDGADP VRRLLAAAGA
GMDLRRAPLI DVHVSDRPVD GRWLALLRIH HLVQDRTTQD VLLGEIHAIL TGREDELPEP
PPFRDFVAQA RLGVPREEHE RYFADLLGDV EETTAPYGLL DVHGDGRGIE RASLAVDEEI
AARVREVARA LRTSPATIFH LAWARVLATL SGRDDVVFGT VLLGRMNTGA DRVPGLFINT
LPVRVRVGGT GAGEALEALR GQLAELLVHE HAPLSLAQRA SGVPGGSPLF TALFDFRHHH
RSGAPDQRDA VRTPGIGTVY TPAEGSTNYP VTVLVDDLGT GFALTVNAAA EAGPEICRML
HACLRNLVTA LENDPADRLS SVRVLDEAER PEDRDDAVPA VATTVTELVE AWAARTPDAV
AVSAGGVEVS YAELDARANR LAHFLRGQGV GAESVVGVCL PRGIELVTAL LGTWKAGAAY
VPIDPGQPAE RIAFVQADSG AVLTLTSEEV LEDLPAGRHR LVAMDDAPTA TRLAGMPGRA
PRVPAPPDQA AYVIYTSGST GRPKGVLVSQ RSAAAYLGWA ARAYPGLGGT AGLHSGVAFD
LAVTSLWGPL AAGGRVRIID LTDDAGDAPA CTFLKVTPTH LAMLDGLMRG FAGDLVVGGE
ALPGGVVERW RAANPAATVV NEYGPTEATV GCVISAAAPG ETVPVGGDGG VLIGRPAPGV
RTFVLDGFLQ AVPAGVAGEL YVAGDQLARG YAGRAGLTAE RFVACPSGGR MYRTGDLVRS
AGDGLLEFLG RVDDQVKVRG FRVEPGEVEG VLSGHPLVAQ AAVVARDERL VAYVVGDADG
DELRRFAAAK LPDYMVPSAF AALDALPLTR GGKLDRKALP APDYAAASTA ATGRAPANAA
EETLCRAFGR VLGLERAGVD DDFFALGGHS LLATKLVGEV RAELGVELPI RQVFSTPTPA
GLAAWIAEHG AGRRTARPAL RRMHAQEESS
//
