ID   A0A1I6J6C9_9MICO        Unreviewed;       602 AA.
AC   A0A1I6J6C9;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN   ORFNames=SAMN05428970_1736 {ECO:0000313|EMBL:SFR74533.1};
OS   Agromyces sp. CF514.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Agromyces.
OX   NCBI_TaxID=1881031 {ECO:0000313|EMBL:SFR74533.1, ECO:0000313|Proteomes:UP000199174};
RN   [1] {ECO:0000313|EMBL:SFR74533.1, ECO:0000313|Proteomes:UP000199174}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CF514 {ECO:0000313|EMBL:SFR74533.1,
RC   ECO:0000313|Proteomes:UP000199174};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000256|ARBA:ARBA00009922}.
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DR   EMBL; FOZD01000001; SFR74533.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I6J6C9; -.
DR   STRING; 1881031.SAMN05428970_1736; -.
DR   OrthoDB; 9806690at2; -.
DR   Proteomes; UP000199174; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   CDD; cd17932; DEXQc_UvrD; 1.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF45; ATP-DEPENDENT DNA HELICASE UVRD2; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}.
FT   DOMAIN          10..290
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   DOMAIN          291..554
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51217"
FT   REGION          319..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         31..38
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   602 AA;  65693 MW;  9558F725A14EEBCC CRC64;
     MSAASDPLLA DLDDEQRVAA EALLGPVCIL AGAGTGKTRA ITHRIAYGVA TGTYDPARVM
     ALTFTSRAAA ELRERLRVLG ADGVPVRTFH AAALAQLNHF WPLVVGGSAP RVLEYKGRLL
     GAAAESIRLR VDQPTLRDVA AEIEWRKVGM LTPEAHDARL AVRGAPGSLS AEQFHDLVAA
     YERIKDERRQ LDFEDVLLAT SGMIEAEASV TMRVHESYRH FVVDEYQDVS PAQQHLLELW
     LGDRDELCVV GDASQTIYSF AGATPEYLLG FERRFADATV VRLERNYRST EPIVDAANRL
     MRGRAGALQL RATARDRMAG EGATVPDAAA PRTSSGHPRG EEPVITSYAD ELAEARGVAT
     VIRNRIDAGA APEQIAVLMR VNSQSAMLEH ALDEVGVASR VRGSVRFFDQ PEVRQAVQAL
     RAAALTIANE PLFKSVSDVL RSLGWTAQAP EGPGAVRARW ESLNALARLV DDAPAGMTFR
     AFADDLRSRA EAQHEPTVQA VTLATLHSAK GLEWDEVHIV GLTEGMLPIA YAKGLDAIDE
     ERRLFYVGVT RARRRLSLSW ARLGGQQRAV REESRFLQEL DSRTTGAARV RDGDRRNPVG
     RT
//