ID A0A1I6KGS9_9BURK Unreviewed; 611 AA.
AC A0A1I6KGS9;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=SAMN05428960_2982 {ECO:0000313|EMBL:SFR90452.1};
OS Mitsuaria sp. PDC51.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Sphaerotilaceae; Roseateles.
OX NCBI_TaxID=1881035 {ECO:0000313|EMBL:SFR90452.1, ECO:0000313|Proteomes:UP000199246};
RN [1] {ECO:0000313|EMBL:SFR90452.1, ECO:0000313|Proteomes:UP000199246}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PDC51 {ECO:0000313|EMBL:SFR90452.1,
RC ECO:0000313|Proteomes:UP000199246};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10060, ECO:0000256|RuleBase:RU361166}.
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DR EMBL; FOZE01000002; SFR90452.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I6KGS9; -.
DR STRING; 1881035.SAMN05428960_2982; -.
DR Proteomes; UP000199246; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02850; E_set_Cellulase_N; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004197; Cellulase_Ig-like.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF02927; CelD_N; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Reference proteome {ECO:0000313|Proteomes:UP000199246};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 25..611
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5011332182"
FT DOMAIN 43..121
FT /note="Cellulase Ig-like"
FT /evidence="ECO:0000259|Pfam:PF02927"
FT DOMAIN 135..603
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 582
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 591
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 611 AA; 64638 MW; 9E266E8F316983C5 CRC64;
MRTRTLAVLA GLAIVGMGAT AVYAQAGDAT KTPAATDTSK AAATPRIEVN QVGYLPAAAK
RATVPAGAAK RFQLIDADSG RAAWTGPLGA AALWAPSRSQ VRIADFSAVT KPGRYRLRVA
GLPDSDPFTI GPGAYDALAA GAIRAFYFNR AGIALTPDLA GPFARPAGHP DTRVLIHASA
AGPKRKTGDV ISSPKGWYDA GDYNKYIVNS GITVYTLLAS YEQMPDRMRA LKVGLPESGN
GLPDVLNEAL WNLDWMLTMQ DPDDGGVYHK LTNPGFDGVV MPDAATTSGD RVVVQKSTAA
ALDFAAVMAI GSRVFKDFER ERPGLSARML AAARAAWGWA QAHPDAIYRQ PPDIHTGDYG
DPTLDDEFAW AAAELYITTG DDAFYTAMKP AQTTMTVPSW GDVRGLAWMS LAAHRDRLGP
AADRALIERR VRELADALVA KGKDSAWGVA LEEKDFNWGS NSNALNQAMV LLQADRLDAA
RPAAAETSAR AGRRAGPTVS AYRQAAQGLL DYVMGRNPTG LSMVTGFGTR SPMHPHHRPS
EADGVVAPVP GFVVGGANAG QQDLKGCPVP YPSPLPALSY LDHFCSYASN EVAINWNAPL
VYVAAALGAP R
//
