ID   A0A1I6LWB6_9MICO        Unreviewed;       312 AA.
AC   A0A1I6LWB6;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 15.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000256|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000256|HAMAP-Rule:MF_01875};
GN   ORFNames=SAMN04487846_2281 {ECO:0000313|EMBL:SFS07757.1};
OS   Microbacterium sp. cf046.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=1761803 {ECO:0000313|EMBL:SFS07757.1, ECO:0000313|Proteomes:UP000199422};
RN   [1] {ECO:0000313|EMBL:SFS07757.1, ECO:0000313|Proteomes:UP000199422}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CF046 {ECO:0000313|EMBL:SFS07757.1,
RC   ECO:0000313|Proteomes:UP000199422};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000256|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000256|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000256|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; FOZJ01000002; SFS07757.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I6LWB6; -.
DR   STRING; 1761803.SAMN04487846_2281; -.
DR   OrthoDB; 9795084at2; -.
DR   Proteomes; UP000199422; Unassembled WGS sequence.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   NCBIfam; TIGR02772; Ku_bact; 1.
DR   PANTHER; PTHR41251; NON-HOMOLOGOUS END JOINING PROTEIN KU; 1.
DR   PANTHER; PTHR41251:SF1; NON-HOMOLOGOUS END JOINING PROTEIN KU; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SPOC domain-like; 1.
PE   3: Inferred from homology;
KW   DNA damage {ECO:0000256|HAMAP-Rule:MF_01875};
KW   DNA recombination {ECO:0000256|HAMAP-Rule:MF_01875};
KW   DNA repair {ECO:0000256|HAMAP-Rule:MF_01875};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW   Rule:MF_01875}; Reference proteome {ECO:0000313|Proteomes:UP000199422}.
FT   DOMAIN          52..179
FT                   /note="Ku"
FT                   /evidence="ECO:0000259|SMART:SM00559"
FT   REGION          228..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          258..312
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   312 AA;  34402 MW;  755EA6DD70CB6A12 CRC64;
     MRAIWKGSLT FGLVNVPVKV YSATEDHDVP LHQVHNKDGG RIRYQRICEI DGEVVPFSDI
     DKAYDDGERT VVLTKEDIDS LPAERSREID VVEFVPSDQV DLLTLDRAYY LEPDSNSPKA
     YVLLRKTLEQ TDRTAIVRFS LRQKTRLAAL RVRDDVLVLQ TLLWADEVRE ATFPALDEPV
     RISAKELEMS ASLVESFAAD FDPSEFSDEY QDELRTLIEA KLEKGDALDT SETFGEHEEE
     AKGGEVIDLM EALRASVERS RAARAGGSSG AKDAEPKAAA KKPAAKSDAA AKAPAKKPAA
     KKAPAKKTAK AS
//