ID A0A1I6MMR6_9MICO Unreviewed; 562 AA.
AC A0A1I6MMR6;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=SAMN04487846_3420 {ECO:0000313|EMBL:SFS16993.1};
OS Microbacterium sp. cf046.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=1761803 {ECO:0000313|EMBL:SFS16993.1, ECO:0000313|Proteomes:UP000199422};
RN [1] {ECO:0000313|EMBL:SFS16993.1, ECO:0000313|Proteomes:UP000199422}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF046 {ECO:0000313|EMBL:SFS16993.1,
RC ECO:0000313|Proteomes:UP000199422};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; FOZJ01000003; SFS16993.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1I6MMR6; -.
DR STRING; 1761803.SAMN04487846_3420; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000199422; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000199422}.
FT DOMAIN 3..271
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 24..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 562 AA; 60939 MW; D8FC46216ADED4F4 CRC64;
MSVEPTALQL QVRDTPDLGL LVVAPAGCGK SEALALRVQG MIRRGVAAGP RRILVATFSN
RAKDNIRERL RSYLTAAELR DKVTVINFHG LASRLYRSHA AAIGLDPEWE LPENDWVKEQ
CQRMGLIYGQ ISAVDKALRQ AKQDMVDDDE VMRRLEALGN QDAIEVERVR IKDQQLTYDD
LPRMAELILA NDAVAGLYSA HFAAVVVDEF QDLTPQQLRM INRIGAGRTT YAGDLAQGIY
GFTGAKPDEI LAAIVAEVAT VVELTESHRS SPAVLAAVNA LTPLTLGSEL ACAAPDTWPS
GGIAAEVHHQ SADSEAEWLV KLAKAIMSRA PGQRIGVISR IGSRRRFADA ALAAESGFEV
HRWDDGVLDT ETARRVRSTL ANVDSAAVLT SKDPIEALRD LADFHELQDP ADRLALADAL
GWMLELLTQG LAPTDIAKRV RVGDNSTLLT RPGVHLLSGH AGKGQQFDWV FTIGVEDGNV
PFFEAKTPEA LLEEARVLSV MMSRARHGVV LSHATAVPAL SGTVWSKDPS RFLTAGLRAQ
LTDAAGLVEW FKAADWEAVA GR
//
