ID   A0A1I6RH43_9ACTN        Unreviewed;       830 AA.
AC   A0A1I6RH43;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Trehalose and maltose hydrolase (Possible phosphorylase) {ECO:0000313|EMBL:SFS63788.1};
GN   ORFNames=SAMN05444716_10362 {ECO:0000313|EMBL:SFS63788.1};
OS   Streptomyces harbinensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1176198 {ECO:0000313|EMBL:SFS63788.1, ECO:0000313|Proteomes:UP000198873};
RN   [1] {ECO:0000313|Proteomes:UP000198873}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.7047 {ECO:0000313|Proteomes:UP000198873};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 65 family.
CC       {ECO:0000256|ARBA:ARBA00006768}.
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DR   EMBL; FPAB01000003; SFS63788.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I6RH43; -.
DR   STRING; 1176198.SAMN05444716_10362; -.
DR   Proteomes; UP000198873; Unassembled WGS sequence.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.70.98.40; Glycoside hydrolase, family 65, N-terminal domain; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR005195; Glyco_hydro_65_M.
DR   InterPro; IPR005196; Glyco_hydro_65_N.
DR   InterPro; IPR037018; Glyco_hydro_65_N_sf.
DR   InterPro; IPR017045; Malt_Pase/Glycosyl_Hdrlase.
DR   PANTHER; PTHR11051; GLYCOSYL HYDROLASE-RELATED; 1.
DR   PANTHER; PTHR11051:SF8; PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE; 1.
DR   Pfam; PF03632; Glyco_hydro_65m; 1.
DR   Pfam; PF03636; Glyco_hydro_65N; 1.
DR   PIRSF; PIRSF036289; Glycosyl_hydrolase_malt_phosph; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000313|EMBL:SFS63788.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198873};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          17..280
FT                   /note="Glycoside hydrolase family 65 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03636"
FT   DOMAIN          332..727
FT                   /note="Glycoside hydrolase family 65 central catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF03632"
FT   REGION          415..434
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        508
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-50"
FT   BINDING         369..370
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
FT   BINDING         628..629
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
SQ   SEQUENCE   830 AA;  93015 MW;  9966CE741783F71C CRC64;
     MTSIELSRLT DEWIWAYEDY DPRSERLREA LCTLGNGYFA TRGAAPEVTA GAAHYPGTYV
     AGCYNRLTSH VAGRDVENED IVNLPNWLPL RFRIHPDDGP PGRWFSPDRE RDGLLLYRQW
     LDLRQGTLHR ALRFRDAHGR HLGVEQTRLV HMGDPHLAAL RTTFTAEDWH GTVEVDSAID
     GTVANNGVAR YRDLAGAHLT KVRTGAAPDA ADTVWLHCRT TRSAIHVALA ARTRCRAGRR
     KPEQVLTKLA GRRAAQRLTV PIGPKKPVTV EKTVALHTSR DRAISGPLEA ALERVRHAGD
     FNTLLGSHAA AWERLWRRAD VEAPGEAGHI LRLHLFHVLQ TLSPHTADLD VGVPARGLHG
     EAYRGHVFWD ELFVLPYLNL HFPEVSRALL TYRYRRLPQA LRAARAIGRD GARFPWQSGS
     DGREETPEVH LNPRSGRWLP DHSRLQHHVG SAIAYNVWRY GQATGDTGFL HAEGAEMLVQ
     IARFWADSAV FDDDLGRYRI KQVVGPDEYH EGYPDADRPG LDDNAYTNVT AAWVLARALE
     LTRELPASRR DDLFARLDMR PGETELWDEV SRRLYVPFHR GVVSQFAGYG ELAELDWEGL
     RARHGDIRRL DRILEAEGDS VNRYQASKQA DVLMLGYLFT PAELGRLFGR LGYDLDEATW
     RRTVDYYLAR TSHGSTLSGL VHAWVLARAR HRDAWEQLQE ALASDVADIQ GGTTAEGIHL
     GAMAGTLDLL QRGLTGLEAR DGVLWMDPAP LPELSEYGFS VRYRSDGGIQ VRMKPGQLWV
     GVPPWEEEPV RVQLADGYVA RDGAGPRDGV GFEVAPGENR LMPLRESTLS
//