ID   A0A1I7HES4_9GAMM        Unreviewed;      1166 AA.
AC   A0A1I7HES4;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN   ORFNames=SAMN04487955_104206 {ECO:0000313|EMBL:SFU59142.1};
OS   Halomonas korlensis.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC   Halomonadaceae; Halomonas.
OX   NCBI_TaxID=463301 {ECO:0000313|EMBL:SFU59142.1, ECO:0000313|Proteomes:UP000198693};
RN   [1] {ECO:0000313|Proteomes:UP000198693}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 1.6981 {ECO:0000313|Proteomes:UP000198693};
RA   Varghese N., Submissions S.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FPBP01000004; SFU59142.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I7HES4; -.
DR   STRING; 463301.SAMN04487955_104206; -.
DR   OrthoDB; 9803237at2; -.
DR   Proteomes; UP000198693; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR048472; DNA_pol_IIIA_C.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR   NCBIfam; TIGR00594; polc; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   4: Predicted;
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198693};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          6..73
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
SQ   SEQUENCE   1166 AA;  130065 MW;  A7274D97C003B0B0 CRC64;
     MTTPFVHLRV HTEYSLVDGL VRLKPLVKAA GEQGSPALAV TDEANLFGLV KFYKTAQGAG
     LKPIIGADLW LANPHDAAHP YRLTLLAMNA AGYRNLTELI SRGWLEGQRQ GRALLDKAWV
     LEQSDGLIAL SGGRDGEIGR HLLADHHDEA RVLLEEWSAA FPGRFYLELI RTGRLQEEEC
     VHLSVDLALE TDTPVVATND VRFLERDDFW AHETRVAIGD GKALDDRRRE RHYTEEQYLK
     SPAEMAELFA DIPEALENSV MIATRCNVDV RLGEIFLPEF AIPEGMTQDE FFRKVSHDGL
     TERLDFLFPP ARYPRDGDEY AEIDKRYRQR LDFELDIILQ MGFPGYFLIV MDFIQWAKDN
     DVPVGPGRGS GAGSLVAYAQ KITDLDPIGY DLLFERFLNP ERVSMPDFDV DFCMEKRDRV
     IDYVAERYGR DAVSQIVTFG TMAAKAVVRD VARAQGKPYS LGDKLSKLIP FEVGMTLAKA
     IDQEPALKEF LEADEDAQEI WEMARRLEGI TRGTGKHAGG VVIAPTKLTD FSPLLCDEDG
     SGLMVQFDKN DIEEAGLVKF DFLGLRTLTI IDWALEMVDK VRSVEGQGPL DIDTIPLDDA
     PTFEMLKKAE TTAVFQLESR GMKELIKRLL PDSLEDMIAL VALFRPGPLQ SGMVDDFINR
     KHGRAEISYP HPDYQHELLK PVLGPTYGII LYQEQVMQIA QVMAGYSLGQ ADLLRRAMGK
     KKPEEMAKQR DGFMEGCAAN GIDRDLAGNL FDLVEKFAGY GFNKSHSAAY ALVSYQTAWL
     KAHYPGPFMA AVMSTEMDNL DKVVPLIEEC RHQGLTVTPP DVNVGSYKFS VDDQGRVVYG
     LGAIRGVGEG PIGAIVEARQ LDGPFADIFD FCRRVDPRRM NKRTLEALIR SGALDTLGPS
     RAVLAAALED ALKAAAQNQS NQNQGMIDMF GEAFVDEASD DAYADYRGAR EWTDKERLAG
     EKDTLGLYLT GHPIDEYEHE LARFVSTRIS DLKPSREPQR VAGLVVGMRT MKSKRGDTMA
     FVTLDDRTGR IEASLFGELY HQLRGRIEPD QVLIVEGEVS SDDYSGGLRL RGKEITPMVA
     ARARYGQAVE LSLDGALANG RLIDSLRASL APWCDAEGLP VRLRYRNAEA SGWLELDEAW
     KVTPSDELLI SLREVQGQEG VRLKYR
//