ID   A0A1I7KR10_9BACT        Unreviewed;       848 AA.
AC   A0A1I7KR10;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   24-JAN-2024, entry version 17.
DE   RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN   ORFNames=SAMN04487941_4018 {ECO:0000313|EMBL:SFU99892.1};
OS   Pontibacter akesuensis.
OC   Bacteria; Bacteroidota; Cytophagia; Cytophagales; Hymenobacteraceae;
OC   Pontibacter.
OX   NCBI_TaxID=388950 {ECO:0000313|EMBL:SFU99892.1, ECO:0000313|Proteomes:UP000182491};
RN   [1] {ECO:0000313|Proteomes:UP000182491}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18820 {ECO:0000313|Proteomes:UP000182491};
RA   Varghese N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|RuleBase:RU361166};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000256|PROSITE-ProRule:PRU10060, ECO:0000256|RuleBase:RU361166}.
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DR   EMBL; FPCA01000007; SFU99892.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1I7KR10; -.
DR   STRING; 388950.GCA_001611675_04082; -.
DR   Proteomes; UP000182491; Unassembled WGS sequence.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd02850; E_set_Cellulase_N; 1.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR004197; Cellulase_Ig-like.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF02927; CelD_N; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW   Glycosidase {ECO:0000256|PROSITE-ProRule:PRU10060,
KW   ECO:0000256|RuleBase:RU361166};
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU10060,
KW   ECO:0000256|RuleBase:RU361166};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Reference proteome {ECO:0000313|Proteomes:UP000182491};
KW   Signal {ECO:0000256|RuleBase:RU361166}.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000256|RuleBase:RU361166"
FT   CHAIN           29..848
FT                   /note="Endoglucanase"
FT                   /evidence="ECO:0000256|RuleBase:RU361166"
FT                   /id="PRO_5010004074"
FT   DOMAIN          284..367
FT                   /note="Cellulase Ig-like"
FT                   /evidence="ECO:0000259|Pfam:PF02927"
FT   DOMAIN          450..836
FT                   /note="Glycoside hydrolase family 9"
FT                   /evidence="ECO:0000259|Pfam:PF00759"
FT   ACT_SITE        818
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        827
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ   SEQUENCE   848 AA;  95040 MW;  91DFEA4669E98579 CRC64;
     MIFTISMKRR IFLSAIVLLL AAFNYASSQD LKLNDLAYFE TQGVNVLVYN NLFTGGFNDE
     KNAGIELIHH GVRTAQGGAV RLSNTPEQWD LVPNISNRKV DRAAKTIEAT LRYEEYNFDS
     RVVVTAKGKG VEIAVYLDKP LPKVLEGAAG FNLEFLPSQY WNKAYLMDGR PNRFPRYVVG
     NTVTKPNSEK PKQFKGFVTS DDRGTGKYID PLPLETGRTF LLAPDEPERL VKITSPDADL
     MLFDGRMLAQ NGWFVVRSLL PAGKTGKVLT WTVEPNAIKG WKREPNIGFS QVGYLPSQPK
     VAVIELDKKD KPLSKSTIYK VGTDGKTTKV FSGKITSWGD YYKYHYVKFD FSSVKAPGIY
     YIQYGDIKTN NFLIENNVYD KITDATSDIW IPIHMNHMFV NEGYRVWHGE PFKEGYLQAP
     PNSDHFDLHA QGPTTDTKYK ALELIPGLNV GGFFDAGDFD IETGSNINVV QNFVHTWEYF
     KPQRDQTFVS QKQRYVDLHR PDGTPDMLQF IEHGTLNLVA QAEIIGHMTQ TLSNAVLDNY
     HHLGDAASST DGLPYNPNLG PYEVAPDGQS SGVKDDLWAF TSRNPSLDLR AATMLAAASR
     ALKGYNDDLS ARALEQSKRL LKEATELLAN QPEDTSRWSR AANVGTNLQL YIATGEQEYK
     DKFQELLWPA LDRGVTFQLL TALNAIPHMD ASYKEKLRPY VVKYNDYIKG LEKDNPYGVP
     IDLGNWAGSG AVTNFGTTVC FANKYFPDVI SASHAFKATN YLFGAHPYHN YSLVATVGAT
     RPKAVFYGNN RADFSFIPGN VAPGVLFRKP DHFENYDDWP FLWGQNEGTI AGNTSYLIFG
     SAFKNLVK
//