ID A0A1I7V5T4_LOALO Unreviewed; 906 AA.
AC A0A1I7V5T4; A0A1S0U841;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Staphylococcal nuclease domain-containing protein {ECO:0000256|PIRNR:PIRNR017179};
DE EC=3.1.31.1 {ECO:0000256|PIRNR:PIRNR017179};
GN ORFNames=LOAG_02162 {ECO:0000313|EMBL:EFO26324.1,
GN ECO:0000313|WBParaSite:EN70_10193};
OS Loa loa (Eye worm) (Filaria loa).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Loa.
OX NCBI_TaxID=7209 {ECO:0000313|Proteomes:UP000095285, ECO:0000313|WBParaSite:EN70_10193};
RN [1] {ECO:0000313|EMBL:EFO26324.1, ECO:0000313|Proteomes:UP000095285}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RG Broad Institute Genome Sequencing Center for Infectious Disease;
RA Nutman T.B., Fink D.L., Russ C., Young S., Zeng Q., Gargeya S.,
RA Alvarado L., Berlin A., Chapman S.B., Chen Z., Freedman E., Gellesch M.,
RA Goldberg J., Griggs A., Gujja S., Heilman E.R., Heiman D., Howarth C.,
RA Mehta T., Neiman D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., White J., Yandava C., Haas B., Henn M.R.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Loa loa.";
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|WBParaSite:EN70_10193}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (NOV-2016) to UniProtKB.
CC -!- FUNCTION: Endonuclease that mediates miRNA decay of both protein-free
CC and AGO2-loaded miRNAs. {ECO:0000256|PIRNR:PIRNR017179}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-
CC phosphooligonucleotide end-products.; EC=3.1.31.1;
CC Evidence={ECO:0000256|PIRNR:PIRNR017179};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|PIRNR:PIRNR017179}.
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DR EMBL; JH712177; EFO26324.1; -; Genomic_DNA.
DR RefSeq; XP_003137748.1; XM_003137700.1.
DR AlphaFoldDB; A0A1I7V5T4; -.
DR STRING; 7209.A0A1I7V5T4; -.
DR EnsemblMetazoa; EFO26324.1; EFO26324.1; LOAG_02162.
DR GeneID; 9939546; -.
DR KEGG; loa:LOAG_02162; -.
DR WBParaSite; EN70_10193; EN70_10193; EN70_10193.
DR CTD; 9939546; -.
DR eggNOG; KOG2039; Eukaryota.
DR InParanoid; A0A1I7V5T4; -.
DR OMA; EKTCCTV; -.
DR OrthoDB; 375531at2759; -.
DR Proteomes; UP000095285; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016442; C:RISC complex; IEA:UniProtKB-UniRule.
DR GO; GO:0016894; F:endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters; IEA:UniProtKB-EC.
DR GO; GO:0004521; F:RNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:InterPro.
DR CDD; cd00175; SNc; 2.
DR CDD; cd20433; Tudor_TDRD11; 1.
DR Gene3D; 2.30.30.140; -; 1.
DR Gene3D; 2.40.50.90; -; 5.
DR InterPro; IPR016685; Silence_cplx_Nase-comp_TudorSN.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR InterPro; IPR002999; Tudor.
DR InterPro; IPR047386; Tudor_TDRD11.
DR PANTHER; PTHR12302; EBNA2 BINDING PROTEIN P100; 1.
DR PANTHER; PTHR12302:SF2; STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1; 1.
DR Pfam; PF00565; SNase; 4.
DR Pfam; PF00567; TUDOR; 1.
DR PIRSF; PIRSF017179; RISC-Tudor-SN; 1.
DR SMART; SM00318; SNc; 4.
DR SMART; SM00333; TUDOR; 1.
DR SUPFAM; SSF50199; Staphylococcal nuclease; 5.
DR SUPFAM; SSF63748; Tudor/PWWP/MBT; 1.
DR PROSITE; PS50830; TNASE_3; 4.
DR PROSITE; PS50304; TUDOR; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR017179};
KW Reference proteome {ECO:0000313|Proteomes:UP000095285};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 13..159
FT /note="TNase-like"
FT /evidence="ECO:0000259|PROSITE:PS50830"
FT DOMAIN 188..321
FT /note="TNase-like"
FT /evidence="ECO:0000259|PROSITE:PS50830"
FT DOMAIN 333..483
FT /note="TNase-like"
FT /evidence="ECO:0000259|PROSITE:PS50830"
FT DOMAIN 516..657
FT /note="TNase-like"
FT /evidence="ECO:0000259|PROSITE:PS50830"
FT DOMAIN 730..788
FT /note="Tudor"
FT /evidence="ECO:0000259|PROSITE:PS50304"
SQ SEQUENCE 906 AA; 101865 MW; 28E0890CE60F359E CRC64;
MSEQQTQPQQ APSLKRGLVK QVLCGDAVVL QGPPMNGPPK EVTVYLSNVI APRLAKRPTD
AESGKEDEPF AWESREFLRK KLIGQNVVFR CDYTATSGRD HGRIYLGGTN LENSENVTET
CVSEGWVEVR VGRVADEYST KLLELQEAAK AAKKGKWALE EGSAQQHVRH VKWVIENPRA
LVDTFKQQKI KAIVEQVRDG STIRAFLLPD FYYITLMLSG IKAPAIRAGA DGRAEDYAEE
ARYFVECRLL QRDVEIILES TSNQNFVGSV IHPKGNIAEL LLKEGFAKCV DWSIALATSG
PEVLRAAERI AKEKKLRFWR TYQPSNQLSV DKKSFTAKVI EIVMGDALIV QKENGDEIKI
WLSSVRPPRE ENRDTENKIG RQFRPLYDIP YLFEAREFLR KRLVGKKVQI ITDYIQPKTE
QFPAKTCCTV MSGGLNVAEA LVSKGLAKVI RYRSDDDNRS SQYDALLAAE AKAEKSKKGL
FAEKELGDKG SVLRIQELQG DAQRSKQFLP YLQRSGRSEG IVEFIASGSR VRLYVPKETC
LITFLFSGID CPRGARIGPG GKLIGENEPF AEEAAKFTRS KIMQREVEVE VEGMDKSGSF
IGYMFVQTEQ GLCNMSVALV ENGLASVHFT AEKGAYYSQL CVAEEKAKKA KLGIWTKWVD
EEAIVQAEIA SADEKMERAI NYRKVVVTDV QRGNFKFAAQ SVDDGPKLEK MMKELREELR
KKPPVVGAYV PRRGDLCVAR FSVDKLWYRA RVEGIKGKNV DILYIDFGNR EMVDVTSMAA
LPADYAAQPA GAREYQMAFL QMPNDPDHAN NSDIAFEQIL FSVPFMFINI EYRNAGVEHV
TAIVETSDGT RTDVAKTLIA EGHALAEQKR EKRFASLIAE YQETEKIARR EHRNIWEYGD
FTGNEL
//
